MAB5224

Anti-Internexin α Antibody, CT, clone 2E3

culture supernatant, clone 2E3, Chemicon^®

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 2E3, monoclonal
• species reactivity: human
• species reactivity (predicted by homology): mammals
• manufacturer/tradename: Chemicon^®
• technique(s): ELISA: suitable; immunohistochemistry: suitable (paraffin); immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_032727.2
• UniProt accession no.: Q16352
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... INA(9118)

Description

General description

The protein alpha-internexin is a ~66 kDa Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits {Pachter J et al J. Cell Biol 101:1316-22 (1985). It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M anbd NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of a-internexin during neuronal regeneration {McGraw et al 2002}. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

Specificity

Alpha internexin. Localized to the C-terminal 164 amino acids of rat alpha internexin.

Immunogen

Epitope: C-terminus

Full length recombinant rat alpha internexin.

Application

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

This Anti-Internexin Antibody, α, C-terminus, clone 2E3 is validated for use in ELISA, IP, WB, IH(P) for the detection of Internexin.

Western blot: antibody detects a protein band at 55-66kDa, as expected for alpha internexin {Evans, et al, 2001}.

Immunocytochemistry on PC12 and Ntera-2 cells.

Immunohistochemistry on formalin and methanol fixed tissues. Works on paraffin embedded tissue sections.

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by the end user.

Linkage

Replaces: 04-1032

Physical form

Mouse IgG in low serum (1%), tissue culture supernatant containing no preservative.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable