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Sigma-Aldrich

Goat Anti-Mouse IgG Antibody, (H+L) HRP conjugate

1 mg/mL, Chemicon®

Synonym(s):

HRP-Conjugated Goat Anti-Mouse IgG

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.46

biological source

goat

Quality Level

100

conjugate

peroxidase conjugate

antibody form

affinity purified immunoglobulin

antibody product type

secondary antibodies

clone

polyclonal

species reactivity

mouse

manufacturer/tradename

Chemicon®

concentration

1 mg/mL

technique(s)

ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

shipped in

wet ice

target post-translational modification

unmodified

Related Categories

General description

The reagent is an affinity purified antibody from goat. The purified antibody is conjugated to horseradish peroxidase (HRP) and stabilized in buffer.

Specificity

Specific for mouse IgG, heavy and light chain. The cross-reactivities of anti-mouse IgG antibody are tested in an ELISA. Minimum cross-reactivity to human IgG.

Application

ELISA:
1:3,000 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).

Immunohistochemistry/immunocytochemistry:
1:200-1:500 dilution can be used (Bullock, G.R., 1982; Javois, L.C., 1994).

Optimal working dilutions must be determined by the end user.
Research Category
Secondary & Control Antibodies
Research Sub Category
Whole Immunoglobulin Secondary Antibodies
This Goat anti-Mouse IgG Antibody, (H+L) HRP conjugate is validated for use in ELISA, IC, IH, WB for the detection of Mouse IgG.

Quality

Western Blot, chromogenic:
1:200-1:500 dilution can be used (Coligan, J., 1997; Harlow, E., 1988).

Western Blot, chemiluminescent:
1:2,000-1:5,000 dilution can be used (Coligan, J., 1997).

Physical form

Affinity purified
Purified Goat IgG conjugated to horseradish peroxidase in buffer containing liquid in 0.01M PBS, pH 7.1 with 15mg/mL BSA and 0.1% Kathon.

Storage and Stability

The undiluted antibody is stable at 2-8°C for 12 months. Do not store in a diluted format. DO NOT FREEZE.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictograms

Exclamation markEnvironment
GHS07,GHS09

Signal Word

Warning

Hazard Statements

H315,H317,H319,H411

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Antigen expression kinetics and immune responses of mice immunized with noninfectious simian-human immunodeficiency virus DNA.
Hegde, R; Liu, Z; Mackay, G; Smith, M; Chebloune, Y; Narayan, O; Singh, DK
Journal of virology null
Kang Wang et al.
Nature communications, 11(1), 4419-4419 (2020-09-06)
Echovirus 30 (E30), a serotype of Enterovirus B (EV-B), recently emerged as a major causative agent of aseptic meningitis worldwide. E30 is particularly devastating in the neonatal population and currently no vaccine or antiviral therapy is available. Here we characterize
Jingsong Cao et al.
Experimental and therapeutic medicine, 19(1), 741-747 (2020-01-04)
In ABO-incompatible (ABOi) kidney transplantation (KT), antibodies can mediate immunological accommodation or immune rejection, but the mechanism by which B cells are induced to produce antibodies with different functions is still unclear. Previous research established an ABOi kidney cell model
Hong Liu et al.
Oncology reports, 42(6), 2759-2767 (2019-10-23)
MicroRNAs (miRs) are a class of non‑coding small RNAs that have been demonstrated to be involved in the pathogenesis of human cancer. There is even evidence that microRNAs can act as oncogenes or tumor suppressors. Although microRNA expression profiles have
Leonardo Marques da Fonseca et al.
Medicines (Basel, Switzerland), 7(4) (2020-04-12)
Background: Piperine, an amide extracted from the Piper spices, exhibits strong anti-tumor properties. However, its effect on the epithelial-mesenchymal transition (EMT) process has never been investigated. Herein, we evaluate the toxic effect of piperine on lung adenocarcinoma (A549), breast adenocarcinoma
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