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Assay
≥95% (SDS-PAGE)
Quality Level
form
lyophilized
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
impurities
≤1.0 EU/μg protein Endotoxin
shipped in
ambient
storage temp.
−20°C
General description
Recombinant, murine lumican, consisting of amino acids 19-338, fused at the C-terminus to a His•Tag sequence, and expressed in NS0-derived mouse myeloma cells. Lumincan a 40-kDa member of the small leucine-rich repeat proteoglycans (SLRPs) family/Class II subfamily of proteins. It is known for its ability to modulate collagen fibrillogenesis and role in cell proliferation regulation.
Biochem/physiol Actions
Cell adhesion: 50-70% after 50 min at 37°C as measured by the ability of immobilized lumican to supoprt the adhesion of 5 X 10⁴ BCE C/D-1b bovine cornea-derived endothelial cells.
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 0.2 µm filtered PBS.
Reconstitution
Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Reconstitute to 200 µg/ml with sterile PBS.
Other Notes
D′Onofrio, M.F. et al. 2008. Biochem. Biophys. Res. Commun.365, 266.
Nikitovic, D. et al. 2008. IUBMB Life60, 818.
Nikitovic, D. et al. 2008. FEBS J.275, 350.
Ishiwata, T. et al. 2007. Oncol. Rep.18, 537.
Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294.
Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422.
Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532.
Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365.
Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347.
Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.
Nikitovic, D. et al. 2008. IUBMB Life60, 818.
Nikitovic, D. et al. 2008. FEBS J.275, 350.
Ishiwata, T. et al. 2007. Oncol. Rep.18, 537.
Vuillermoz, B. et al. 2004. Exp. Cell Res.296,294.
Chakravarti, S. et al. 2003. Invest. Ophthalmol. Vis. Sci.44, 2422.
Jepsen, K.J. et al. 2002. J. Biol. Chem.277, 35532.
Chakravarti, S. et al. 2000. Invest. Ophthalmol. Vis. Sci.41,3365.
Blochberger, T.C. et al. 1992. J. Biol. Chem.267, 347.
Chakravarti, S. et al. 1998. J. Cell Biol.141, 1277.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
HIS TAG is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 2
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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