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Sigma-Aldrich

Calpastatin Peptide

A 27-amino acid, cell-permeable peptide encoded by exon 1B of human calpastatin that acts as a potent inhibitor of calpain I and calpain II (IC₅₀ = 20 nM for purified rabbit calpain II).

Synonym(s):

Calpastatin Peptide, CS Peptide

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About This Item

Empirical Formula (Hill Notation):
C142H230N36O44S
Molecular Weight:
3177.63
UNSPSC Code:
12352202
NACRES:
NA.77

Quality Level

100

Assay

≥95% (HPLC)

form

lyophilized solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated (hygroscopic)

color

white

solubility

1% acetic acid: 1 mg/mL
water: 1 mg/mL

shipped in

ambient

storage temp.

−20°C

General description

A 27-amino acid, cell-permeable peptide encoded by exon 1B of human calpastatin that acts as a potent inhibitor of calpain I and calpain II (IC50 = 20 nM for purified rabbit calpain II). Does not inhibit either papain or trypsin. Blocks the down-regulation of protein kinase Cε (PKCε) in rat pituitary GH4C1 cells stimulated by thyrotropin-releasing hormone (TRH).
A 27-amino acid, peptide encoded by exon 1B of human calpastatin that acts as a cell-permeable and potent inhibitor of calpain I and calpain II (IC50 = 20 nM for purified rabbit calpain II). Does not inhibit either papain or trypsin. Blocks the down-regulation of protein kinase Cε (PKCε) in rat pituitary GH4C1 cells stimulated by thyrotropin-releasing hormone (TRH).

Biochem/physiol Actions

Cell permeable: yes
Primary Target
calpain 2
Product does not compete with ATP.
Reversible: no
Target IC50: 20 nM against calpain II

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Asp-Pro-Met-Ser-Ser-Thr-Tyr-Ile-Glu-Glu-Leu-Gly-Lys-Arg-Glu-Val-Thr-Ile-Pro-Pro-Lys-Tyr-Arg-Glu-Leu-Leu-Ala-NH₂

Physical form

Supplied as a trifluoroacetate salt.

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.

Other Notes

Eto, A., et al. 1995. J. Biol. Chem. 270, 25115.
Kawasaki, J., et al. 1989. J. Biochem. 106, 274.
Maki, M., et al. 1989. J. Biol. Chem. 264, 18866.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Chanporn Chaosap et al.
Animal bioscience, 34(9), 1514-1524 (2021-04-27)
This study investigated the meat quality characteristics, endogenous proteolytic enzymes, collagen content, and myosin heavy chain (MyHC) isoforms of different muscles of Thai native cattle (TNC). Infraspinatus (IF), Longissimus thoracis (LT), and Supraspinatus (SS) muscles were obtained from two TNC
Xianliang Zeng et al.
Oncology letters, 21(2), 124-124 (2021-02-09)
Cancer cachexia is a life-threatening syndrome characterized by muscle atrophy. Cancer cachectic muscle atrophy (CCMA) is associated with mitochondrial injury. Mitochondrial calpains have been reported to induce mitochondrial injury in mouse cardiomyocytes and pulmonary smooth muscle. In the present study
Li Huang et al.
International journal of molecular sciences, 22(12) (2021-07-03)
Retinitis pigmentosa (RP) is an inherited form of retinal degeneration characterized by primary rod photoreceptor cell death followed by cone loss. Mutations in several genes linked to the disease cause increased levels of cyclic guanosine monophosphate (cGMP) and calcium ion

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