14-842-M

Lck Protein, activated, 10 µg

activated, N-terminal 6His-tagged, recombinant, human Lck full length, for use in Kinase Assays.

• UNSPSC Code: 12352202
• eCl@ss: 32160405
• NACRES: NA.41

Properties

• biological source: human
• Quality Level: 100
• recombinant: expressed in baculovirus infected Sf21 cells
• product line: Upstate^®
• specific activity: (For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.)
• mol wt: M_w 59 kDa
• purified by: (Ni²⁺/NTA-agarose)
• species reactivity: human
• manufacturer/tradename: Upstate^®
• technique(s): activity assay: suitable (kinase)
• accession no.: (FUNCTION: SwissProt: P06239 # Tyrosine kinase that plays an essential role for the selection and maturation of developing T-cell in the thymus and in mature T-cell function. Is constitutively associated with the cytoplasmic portions of the CD4 and CD8 surface receptors and plays a key role in T-cell antigen receptor(TCR)-linked signal transduction pathways. Association of the TCR with a peptide antigen-bound MHC complex facilitates the interaction of CD4 and CD8 with MHC class II and class I molecules, respectively, and thereby recruits the associated LCK to the vicinity of the TCR/CD3 complex. LCK then phosphorylates tyrosines residues within the immunoreceptor tyrosines-based activation motifs (ITAMs) in the cytoplasmic tails of the TCRgamma chains and CD3 subunits, initiating the TCR/CD3 signaling pathway. In addition, contributes to signaling by other receptor molecules. Associates directly with the cytoplasmic tail of CD2, and upon engagement of the CD2 molecule, LCK undergoes hyp); NM_005356.3
• NCBI accession no.: NM_005356.3
• UniProt accession no.: P06239
• relevant disease(s): obesity; metabolic disorders

Description

General description

N-terminal 6His-tagged, recombinant, human Lck full length

Application

Auto-activated on column by incubating with Mg/ATP, excess ATP and MgAc removed by multiple column wash steps.

Research Category
Metabolism

Research Sub Category
Obesity

Metabolic Disorders

Biochem/physiol Actions

Protein Target: Lck

Target Sub-Family: TK

Packaging

Also available in 10 μg size and in bulk

Also available in 250 µg size or bulk --call for pricing and availability and reference catalog number 14-842M when ordering the 250 µg size.

Target description

Lck

Linkage

Replaces: 14-710

Physical form

Ni²⁺/NTA-agarose

Storage and Stability

1 year at -70°C from date of shipment

Other Notes

For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H317
• Precautionary Statements: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(C): Not applicable