116815-M

Adenosine 3′,5′-cyclic Monophosphorothioate, Sp-Isomer, Triethylammonium Salt

A cell-permeable cAMP analog that binds cAMP-receptor proteins.

• Synonym(s): Adenosine 3′,5′-cyclic Monophosphorothioate, Sp-Isomer, Triethylammonium Salt, Adenosine 3ʹ,5ʹ-cyclic Phosphorothioate-Sp, Sp-cAMPS, TEA
• Empirical Formula (Hill Notation): C₁₀H₁₁N₅O₅PS · 4C₆H₁₆N
• CAS Number: 71774-13-5
• Molecular Weight: 753.06
• UNSPSC Code: 12352200

Properties

• Assay: ≥98% (HPLC)
• form: crystalline solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; desiccated (hygroscopic)
• solubility: water: 20 mg/mL
• storage temp.: −20°C

Description

General description

A cell-permeable cAMP analog that binds cAMP-receptor proteins. Induces the guanine-nucleotide exchange factor activity of GEFI (also known as Epac) and GEFII towards Raf1, both in vivo and in vitro. Known to cause a decrease in cytosolic pH. Note: 10 µmol = 4.47 mg.

A cell-permeable cAMP analog that binds cAMP-receptor proteins. Induces the guanine-nucleotide-exchange factor activity of GEFI, also known as Epac (exchange protein directly activated by cAMP), and GEFII towards Rap1 both in vivo and in vitro. Known to cause a decrease in cytosolic pH. Note: 10 µmol = 4.47 mg. λ_max = 258 nm; ε_max = 15,000 M^-1cm-1 (pH 7..0).

Note: 10 µmol = 4.47 mg.

Biochem/physiol Actions

Primary Target
cAMP-receptor proteins

Warning

Toxicity: Standard Handling (A)

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Aqueous stock solutions are stable for up to 1 month at -20°C.

Other Notes

Kawasaki, H., et al. 1998. Science 282, 2275.
de Rooij, J., et al. 1998. Nature 396, 474.
Kaji, H., et al. 1992. Biochem. Biophys. Res. Commun.182, 1356.
Sugimoto, T., et al. 1992. J. Cell Physiol. 152, 28.
Van Der Kloot, W., and Branisteanu, D.D. 1992. Pflugers Arch.420, 336.
Takuma, T., and Ichida, T. 1991. J. Biochem.110, 292.
Dostmann, W.R., et al. 1990. J. Biol. Chem.265, 10484.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable