sensitivity: 0.49-500 pg/mL accuracy: 80-120% (minimum of 10 samples spiked with acceptable recovery)
technique(s)
ELISA: suitable single molecule counting: suitable
General description
The SMC® Mouse TNF-α Discovery Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The SMC® immunoassay system detects analytes using Single Molecule Counting (SMC®) technology.
Tumor necrosis factor alpha (TNF-α) is a pro-inflammatory cytokine with cytotoxic activity that is primarily produced by macrophages. TNF-α is involved in systemic inflammation and is a member of the group of cytokines that stimulate the acute phase reaction. When administered to animals or humans it causes inflammation, fever, cardiovascular effects, hemorrhage, coagulation and acute phase responses similar to those seen during acute infections and shock states. By promoting the inflammatory response, TNF-α is associated with many autoimmune disorders such as rheumatoid arthritis, ankylosing spondylitis, Crohn′s disease, psoriasis and refractory asthma. TNF-α also plays a role in cancer and regulates apoptotic cell death, cellular proliferation, differentiation, tumorigenesis and viral replication.
Application
Research Category Inflammation & Immunology
Research Sub Category Cytokines & Cytokine Receptors
The sensitivity of Single Molecule Counting (SMC®) technology gives the SMC® Mouse TNF-α Discovery Immunoassay unparalleled performance in the quantification of TNF-α in mouse serum.
Components
Assay Buffer Mouse TNF-α Coating Antibody Assay Plate 10X PBS Blocking Buffer Standard Diluent Mouse TNF-α Detection Antibody Mouse TNF-α Standard SMC® Mouse TNF-α Immunoassay Kit Instructions 10X Wash Buffer Buffer C Elution Buffer B
Analysis Note
Low-end CV% Range: 0-16%|Low-end CV% Average: 6%
Legal Information
SMC is a registered trademark of Singulex
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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