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850534O

Avanti

7.9 MAG

Avanti Research™ - A Croda Brand 850534O

Synonym(s):

1-(7Z-hexadecenoyl)-rac-glycerol

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About This Item

Empirical Formula (Hill Notation):
C19H36O4
CAS Number:
749266-29-3
Molecular Weight:
328.49
UNSPSC Code:
12352211
NACRES:
NA.25

Assay

>99% (TLC)

form

oil

mol wt

328.49 g/mol

packaging

pkg of 10 × 0.1 g (with screw cap (850534O-1g))
pkg of 2 × 100 mg (with screw cap (850534O-200mg))
pkg of 1 × 25 mg (with screw cap (850534O-25mg))

manufacturer/tradename

Avanti Research™ - A Croda Brand 850534O

technique(s)

protein sequencing: suitable

shipped in

dry ice

storage temp.

−20°C

SMILES string

OCC(O)COC(CCCCC/C=C\CCCCCCCC)=O

General description

This new Lipid detergent may be used for crystallizing membrane proteins by using lipidic mesophases. This method has variously been referred to as the lipidic cubic phase or in meso method. The method has been shown to be quite versatile in that it has been used to solve X-ray crystallographic structures of prokaryotic and eukaryotic proteins, proteins that are monomeric, homo- and hetero-multimeric, chromophore-containing and chromophore-free, and alpha-helical and beta-barrel proteins.

Application

7.9 MAG is suitable for use as a hosting lipid for titration to avoid lamellar crystal phase (Lc) formation during sample injection It is also suitable for use in mesocrystallization.

Biochem/physiol Actions

7.9 MAG is a non-monoolein lipid. It can support crystallization of bacteriorhodopsin, an integral membrane protein.

Packaging

5 mL PTFE Vial with Screw Cap (850534O-1g)
5 mL PTFE Vial with Screw Cap (850534O-200mg)
5 mL PTFE Vial with Screw Cap (850534O-25mg)

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

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Description
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Storage Class Code

11 - Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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In Meso Crystallization of the Integral Membrane Glycerol 3-Phosphate Acyltransferase with Substrates
Li Z, et al.
Crystal Growth & Design, 18(4), 2243-2258 (2018)
Membrane protein crystallization in lipidic mesophases with tailored bilayers
Misquitta LV, et al.
Structure, 12(12), 2113-2124 (2004)
Preparation and delivery of protein microcrystals in lipidic cubic phase for serial femtosecond crystallography
Ishchenko A, et al.
Journal of Visualized Experiments, (115), e54463-e54463 (2016)
Niklaus Johner et al.
Journal of the American Chemical Society, 136(8), 3271-3284 (2014-02-06)
The recent advances in the in meso crystallization technique for the structural characterization of G-protein coupled receptor (GPCR) proteins have established the usefulness of the lipidic-cubic phases (LCPs) in the field of crystallography of membrane proteins. It is surprising that
Martin Caffrey et al.
Journal of visualized experiments : JoVE, (45) (2010-11-30)
A detailed protocol for crystallizing membrane proteins by using lipidic mesophases is described. This method has variously been referred to as the lipidic cubic phase or in meso method. The method has been shown to be quite versatile in that

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