New linker phosphoramidite reagents containing a cleavable 3'-ester linkage are used for attaching the first nucleoside to the surface of a solid- phase support. Inexpensive, underivatized amino supports, such as long chain alkylamine controlled-pore glass, can serve as universal supports.
Xenobiotica; the fate of foreign compounds in biological systems, 22(4), 405-418 (1992-04-01)
1. The metabolism of sulphur mustard, 1,1'-thiobis(2-chloroethane), in vivo was investigated following i.p. administration to rat. 2. Approx. 60% of dose was excreted in the 24 h urine. Many metabolites were present; nine have been isolated by h.p.l.c. and characterized
Journal of chromatography. B, Biomedical applications, 665(1), 97-105 (1995-03-10)
Gas chromatography-tandem mass spectrometry (GC-MS-MS) with selected-reaction monitoring was applied to the analysis of urinary metabolites of sulphur mustard, derived from the beta-lyase pathway and from hydrolysis. In the case of beta-lyase metabolites, a limit of detection of 0.1 ng/ml
Xenobiotica; the fate of foreign compounds in biological systems, 25(2), 167-173 (1995-02-01)
1. Samples of urine from two human subjects accidentally exposed to sulphur mustard were analysed for metabolites derived from hydrolysis (thiodiglycol, thiodiglycol sulphoxide), conjugation with glutathione (1,1'-sulphonylbis [2-S-(N-acetylcysteinyl)ethane]) and from further metabolism of glutathione conjugates by the beta-lyase pathway (1,1-sulphonylbis[2-(methylsulphinyl)ethane]
Journal of chromatography, 558(2), 393-404 (1991-10-11)
Two methods have been developed for the analysis of thiodiglycol sulphoxide, a metabolite of sulphur mustard, in urine. The first method recovers thiodiglycol sulphoxide from urine by extraction from a solid absorbent tube and clean up on Florisil. In the
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