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ZRB1280

Sigma-Aldrich

Anti-ARID1A Antibody, clone 1C3 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):

AT-rich interactive domain-containing protein 1A

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1C3, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 242.05 kDa
observed mol wt ~250 kDa

purified by

using Protein A

species reactivity

mouse, human

species reactivity (predicted by homology)

rat, monkey, canine, bovine

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

Internal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ARID1A(8289)

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1C3 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects ARID domain-containing protein 1A (ARID1A). It targets an epitope within 22 amino acids from the internal region. May detect all three isoforms.

Immunogen

KLH-conjugated linear peptide corresponding to 22 amino acids from the internal region of human ARID domain-containing protein 1A (ARID1A).

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate..

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ARID1A in HeLa cell lysate.

Tested applications

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ARID1A in HeLa, A431, HUVEC and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected ARID1A in human colon tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound ARID1A with a KD of 4.3 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

AT-rich interactive domain-containing protein 1A (UniProt: O14497; also known as ARID domain-containing protein 1A, B120, BRG1-associated factor 250, BAF250, BRG1-associated factor 250a, BAF250A, Osa homolog 1, hOSA1, SWI-like protein, SWI/SNF complex protein p270, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily F member 1, hELD) is encoded by the ARID1A (also known as BAF250, BAF250A, C1orf4, OSA1, SMARCF1) gene (Gene ID: 8289) in human. ARID1A is a member of a large family of proteins that contain a characteristic ARID domain of approximately 100 amino acids that binds DNA fragments rich in adenine-thymine (AT). It is a component of SWI/SNF chromatin complexes and is highly expressed in spleen, thymus, prostate, testis, ovary, small intestine, and colon. At lower level it is also expressed in several other tissues. It is involved in transcriptional activation and repression of select genes by chromatin remodeling. It changes chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. ARID1A is shown to be critical for embryonic stem cell pluripotency and their differentiation into mesoderm-derived cardiomyocytes and adipocytes. It is also a component of the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. Nuclear ARID1A is shown to be more unstable than its cytoplasmic counterpart, as it degrades rapidly, by the ubiquitin-proteasome system of the nucleus. It is normally detected during the G0-G1 phase of the cell cycle and its levels decrease during the G2-S phase. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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