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SAB4200148

Sigma-Aldrich

Anti-Derlin-1 antibody, Mouse monoclonal

clone Derlin1-1, purified from hybridoma cell culture

Synonym(s):

Anti-DER-1, Anti-DERL1, Anti-Der1-like domain family, member 1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

Derlin1-1, monoclonal

mol wt

antigen ~22 kDa

species reactivity

mouse, bovine, rat, canine, human

concentration

~1.0 mg/mL

technique(s)

immunoprecipitation (IP): suitable
western blot: 2-4 μg/mL using whole extracts of mouse NIH-3T3 or rat NRK cells

isotype

IgG1

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... DERL1(79139)
mouse ... Derl1(67819)
rat ... Derl1(362912)

General description

Derlin-1 (DERL1) gene with seven exons is mapped to human chromosome 8q24.13. DERL1 is an endoplasmic reticulum (ER) membrane protein and is a key component of p97 ATPase complex.
Monoclonal Anti-Derlin-1 (mouse IgG1 isotype) is derived from the hybridoma Derlin1-1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Derlin-1, a human homolog of yeast Der1p, is a membrane protein.

Immunogen

synthetic peptide corresponding to the C-terminal region of human Derlin-1, conjugated to KLH. The corresponding sequence is identical in mouse, rat, monkey, pig, bovine and canine.

Application

Monoclonal Anti-Derlin-1 antibody produced in mouse has been used in western blotting and immunoprecipitation.

Biochem/physiol Actions

Derlin-1 is a multifunctional protein. It acts as a key growth factor-responsive endothelial antigen that stimulates endothelial cell survival and growth. The encoded protein might also be implicated in endoplasmic reticulum stress in neuronal cells in Alzheimer′s disease.Elevated expression of DERL1 has been observed in various types of cancer including bladder and lung cancer. Additionally, overexpression of the gene is also associated with the development of colon cancers. Thus, DERL1 can be used as a potential therapeutic target for colon cancer. DERL1 plays an essential role in proteostasis regulation of potassium channel (KATP)channels.
Derlin-1 is required for the dislocation of misfolded proteins from the endoplasmic reticulum (ER) lumen to the cytosol. It interacts with peptide:N-glycosidase (PNGase), a deglycosylating enzyme, bringing it close to misfolding dislocating glycoproteins. Derlin-1 interacts with VIMP (VCP-interacting membrane protein), a membrane protein that recruits p97 and its cofactors, ubiquitin recognition factor in ER associated degradation 1 (Ufd1) and nuclear protein localization 4 (Npl4). Derlin-1 and VIMP form a membrane protein complex that serves as a receptor for p97.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A small-molecule inhibitor of SOD1-Derlin-1 interaction ameliorates pathology in an ALS mouse model
Tsuburaya N, et al.
Nature Communications, 9, 2668-2668 (2018)
High Expression of Derlin-1 Is Associated with the Malignancy of Bladder Cancer in a Chinese Han Population.
Wu Z
PLoS ONE, 11 (2016)
Identification of derlin-1 as a novel growth factor-responsive endothelial antigen by suppression subtractive hybridization.
Ran Y
Biochemical and Biophysical Research Communications, 348, 1272-1278 (2006)
Derlin-1-immunopositive inclusions in patients with Alzheimer's disease.
Honjo Y
Neuroreport, 23, 611-615 (2012)
Derlin-2 and Derlin-3 are regulated by the mammalian unfolded protein response and are required for ER-associated degradation
Oda Y, et al.
The Journal of cell biology, 172, 383-393 (2006)

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