P0389

Penicillinase from Bacillus cereus

lyophilized powder, 1,500-3,000 units/mg protein (using benzylpenicillin)

• Synonym(s): β-Lactamase I from Bacillus cereus, β-Lactamase II, Cephalosporinase, Penicillin amido-β-lactam hydrolase
• CAS Number: 9073-60-3
• Enzyme Commission number: 3.5.2.6 (BRENDA, IUBMB)
• MDL number: MFCD00131819
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: Bacillus sp. (Bacillus cereus)
• Quality Level: 300
• form: lyophilized powder
• specific activity: 1,500-3,000 units/mg protein (using benzylpenicillin)
• composition: Protein, ~10%
• technique(s): activity assay: suitable
• application(s): diagnostic assay manufacturing
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Penicillinase from Bacillus cereus has been shown to contain three distinct species when purified and examined for homogeneity by sedimentation analysis, amino-terminal analysis, and vertical acrylamide gel electrophoresis.

Application

Penicillinase can be used to reduce levels of penicillin in patients with severe penicillin reactions.

Used in the production of penicillin.

Used in the production of penicillin. Penicillinase from Bacillus cereus has been used in a comparison study with penicillinase from Bacillus subtillis where the Michaelis constant, shape of the Ph/activity curve and immunological properties were shown to be significantly different.

Biochem/physiol Actions

Penicillinase specifically catalyzes the hydrolysis of β-lactam ring of penicillin.

Unit Definition

One unit will hydrolyze 1.0 μmole of indicated substrate per min at pH 7.0 at 25 °C. Sold as benzylpenicillin units. This International Unit (using benzylpenicillin as substrate) is approximately equal to 600 Levy or 75 Pollock units.

Physical form

Lyophilized powder containing phosphate and citrate buffer salts

Analysis Note

Protein determined by biuret.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H317,H334
• Precautionary Statements: P261 - P272 - P280 - P284 - P302 + P352 - P333 + P313
• Hazard Classifications: Resp. Sens. 1 - Skin Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves