MTOX1004

MDR1/BCRP Double Knockout Caco-2 Cells

Human male colorectal tissue, adenocarcinoma

• Synonym(s): C2BBe1 Cells MDR1/BCRP (-/-/-/-,-/-/-/-)
• UNSPSC Code: 41106514
• NACRES: NA.81

Properties

• product name: MDR1/BCRP Double Knockout Caco-2 Cells, one vial
• biological source: human male colorectal tissue (Source disease: adenocarcinoma)
• Quality Level: 200
• form: liquid
• technique(s): drug transporter assay: suitable; permeability assay: suitable
• OMIM accession no.: 171050; 603756
• application(s): ADME/TOX
• storage temp.: −196°C
• Gene Information: human ... ABCB1(5243) , ABCG2(9429)

Description

General description

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 and BCRP knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 and ABCG2 (MDR1/BCRP) efflux transporters.

Application

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays:

Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases

Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes

Caco-2 Transporter Knockout Cell Based Assays

Features and Benefits

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include:

• A functional double knockout of the MDR1 gene and BCRP gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP2 substrate
• The vial format enables the MDR1 and BCRP knockout cells to be included in standard drug transporter protocols
• Human assay with no interference from animal inhibitors
• Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

Legal Information

ADME/Tox Cell Lines License

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Kit Components Also Available Separately

• MTOX1004 MDR1/BCRP Double Knockout Caco-2 Cells, one vial 1 vial

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable