I9658
Anti-Importin α1 antibody, Rat monoclonal
clone 1A6, purified from hybridoma cell culture
Synonym(s):
Anti-Imp α, Monoclonal Anti-Importin α1 antibody produced in rat
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About This Item
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biological source
rat
Quality Level
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
1A6, monoclonal
form
buffered aqueous solution
mol wt
antigen ~60 kDa
species reactivity
human
technique(s)
immunocytochemistry: suitable
microarray: suitable
western blot: 0.1-0.2 μg/mL
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... KPNA2(3838)
mouse ... Kpna2(16647)
General description
Monoclonal Anti-Importin α1 (rat IgG2α isotype) is derived from the hybridoma 1A6 produced by the fusion of mouse myeloma cells (SP2/0), and splenocytes from BN/Crj rats.
The KPNA2 (karyopherin subunit α 2) consists of 529 amino acids and has a molar mass of 58kDa. The protein consists of an N-terminal hydrophilic importin β-binding domain that has an auto-inhibitory function to ensure the translocation of KPNA2 occurs only when it is bound to importin β and a cargo molecule. It also contains a central hydrophobic region made up of 10 armadillo (ARM) repeats, which binds the cargo′s NLS (nuclear localization signal) and CAS, the factor involved in the recycling of KPNA2 to the cytoplasm. The C-terminus has a short acidic region whose function is yet to be determined. The gene is mapped to human chromosome 17q24.2.
Specificity
Monoclonal Anti-Importin a1 recognizes human, canine, mouse, and chicken Importin a1 (approx. 60kDa)
Immunogen
recombinant mouse importin α1.
Application
Monoclonal Anti-Importin α1 antibody produced in rat has been used in:
- immunoblotting
- immunofluorescence
- immunocytochemistry
Biochem/physiol Actions
The gene KPNA2 (karyopherin subunit α 2), also referred to as importin α1, encodes a member of the importin α family. It participates in the normal nuclear localization of the MRN (MRE11-RAD50-NBS1) complex. It also facilitates proper formation of the nuclear foci of the complex. KPNA2 is the sole member of its family to interact with NBS1 (Nijmegen breakage syndrome 1), a component of the MRN complex. It also functions in the tumor suppression functions associated with NBS1 complex. Inhibition of the interaction between KPNA2 and NBS1 can lead to decreased radiation-induced nuclear focus accumulation, DSB (double strand break) repair, and cell cycle checkpoint signaling of NBS1. KPNA2 is found to regulate epidermal proliferation and differentiation and participates in the activation of cellular signaling in blood lymphocytes. It has been linked to Russell-Silver syndrome. Overexpression of this protein has been associated with several types of cancer and it may serve as a potential biomarker.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.
Storage and Stability
For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Anders Christiansen et al.
Cancer letters, 331(1), 18-23 (2012-12-27)
In recent years, Karyopherin α 2 (KPNA2) has emerged as a potential biomarker in multiple cancer forms. The aberrant high levels observed in cancer tissue have been associated with adverse patient characteristics, prompting the idea that KPNA2 plays a role
Shun-Fu Tseng et al.
The Journal of biological chemistry, 280(47), 39594-39600 (2005-09-29)
Nijmegen breakage syndrome (NBS) is a chromosomal-instability syndrome associated with cancer predisposition, radiosensitivity, microcephaly, and growth retardation. The NBS gene product, NBS1, is a component of the MRE11-RAD50-NBS1 (MRN) complex, a central player associated with double strand break (DSB) repair.
Li He et al.
PloS one, 7(9), e42992-e42992 (2012-09-11)
The aim of this study was to identify a biomarker useful in the diagnosis and therapy of ovarian malignant germ cell tumor (OMGCT). The karyopherin 2 (KPNA2) expression in OMGCT and normal ovarian tissue was determined by standard gene microarray
Li Guo et al.
Journal of cell science, 132(18) (2019-08-23)
Importin-α serves as an adaptor linking importin-β to proteins carrying a nuclear localization sequence (NLS). During interphase, this interaction enables nuclear protein import, while in mitosis it regulates spindle assembly factors (SAFs) and controls microtubule nucleation, stabilization and spindle function.
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