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Key Documents

HPA014753

Sigma-Aldrich

Anti-CYB561 antibody produced in rabbit

Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution

Synonym(s):

Anti-CYB561A1, Anti-FRRS2

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About This Item

UNSPSC Code:
12352203
Human Protein Atlas Number:
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

product line

Prestige Antibodies® Powered by Atlas Antibodies

form

buffered aqueous glycerol solution

species reactivity

human

technique(s)

immunohistochemistry: 1:50-1:200

immunogen sequence

RADWKRPSQAEEQALSMDFKTLTEGDS

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CYB561(1534)

General description

CYB561 (cytochrome b561) is a member of di-heme, transmembrane protein family, and are found in both animals and plants. It has six transmembrane α-helices. The central four make up the core of this protein, containing two heme b molecules, with four conserved histidine residues. It acts as electron shuttle, found in the membrane of storage vesicles. It has a molecular weight of ~30kDa, and is a monomer composed of 251 amino acids.

Immunogen

Cytochrome b561 recombinant protein epitope signature tag (PrEST)

Application

All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project and as a result, are supported by the most extensive characterization in the industry.

The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. We also provide Prestige Antibodies® protocols and other useful information.

Biochem/physiol Actions

CYB561 (cytochrome b561) is responsible for the regeneration of ascorbate, by functioning as monodehydroascorbate reductase. It is also involved in transmembrane transport, where it acts as Fe3+-reductase, and reduces iron. It also plays a role in defense against stress, modifications of cell wall, neurological processes such as memory formation, metabolism of iron and suppression of tumor. It is involved in catecholamine synthesis, where it acts as storage vesicle membrane electron shuttle. It is the source of reduced equivalents to two monooxygenases- peptidylglycine α-amidating monooxygenase in neurosecretory vesicles and dopamine β-hydroxylase in chromaffin granules. Polymorphism in this gene is associated with inherited changes in both basal and stress-related heart rate.

Features and Benefits

Prestige Antibodies® are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies® to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.

Every Prestige Antibody is tested in the following ways:
  • IHC tissue array of 44 normal human tissues and 20 of the most common cancer type tissues.
  • Protein array of 364 human recombinant protein fragments.

Linkage

Corresponding Antigen APREST73253

Physical form

Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide

Legal Information

Prestige Antibodies is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Kuixing Zhang et al.
Journal of the American College of Cardiology, 63(4), 358-368 (2013-10-22)
The goal of this study was to understand the role of genetic variation in the catecholamine biosynthetic pathway for control of human heart rate (HR). Human HR is an integrated cardiovascular trait predictive of morbidity and survival. Because the autonomic
Han Asard et al.
Antioxidants & redox signaling, 19(9), 1026-1035 (2012-12-20)
Cytochromes b561 (CYB561s) constitute a family of trans-membrane (TM), di-heme proteins, occurring in a variety of organs and cell types, in plants and animals, and using ascorbate (ASC) as an electron donor. CYB561s function as monodehydroascorbate reductase, regenerating ASC, and
Maple M Fung et al.
Circulation, 117(4), 517-525 (2008-01-09)
Catecholamines govern stress blood pressure responses. Catecholaminergic responses may be partially genetic and contribute to the complex heritability of hypertension. To evaluate catecholaminergic responses without systemic counterregulation, we infused graded concentrations of tyramine, an indirect presynaptic norepinephrine releaser, into dorsal
Romie Angelo G Azur et al.
PloS one, 19(5), e0300413-e0300413 (2024-05-13)
Castration-resistant prostate cancer (CRPC) is associated with resistance to androgen deprivation therapy, and an increase in the population of neuroendocrine (NE) differentiated cells. It is hypothesized that NE differentiated cells secrete neuropeptides that support androgen-independent tumor growth and induce aggressiveness

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