Skip to Content
Merck
All Photos(1)

Documents

H1506

Sigma-Aldrich

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosteroni

≥15 units/mg protein

Synonym(s):

3α-Hydroxysteroid:NAD(P)+ oxidoreductase

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

CAS Number:
9028-56-2
Enzyme Commission number:
1.1.1.50 (BRENDA, IUBMB)
EC Number:
232-839-5
MDL number:
MFCD00130298
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

bacterial (Pseudomonas testosteroni)

Quality Level

200

form

lyophilized solid

specific activity

≥15 units/mg protein

purified by

chromatography

composition

Protein, ≥25%

foreign activity

β-hydroxysteroid dehydrogenase ≤0.5%
alcohol dehydrogenase ≤0.05%

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Application

3α-Hydroxysteroid Dehydrogenase from Pseudomonas testosterone has been used:
  • in the preparation of composite electrode platform to for the determination of androsterone
  • in potassium phosphate buffer for the bioluminescent assay of bile acids in serum
  • for total bile salts assay using an enzymatic method

Biochem/physiol Actions

3α-hydroxysteroid dehydrogenase (3α-HSD) may help to modulate the occupancy of the androgen receptor (AR) in androgen target tissues. It may also act in hindering androgen action.

Unit Definition

One unit will oxidize 1.0 μmole of androsterone per min at pH 8.9 at 25 °C in the presence of β-NAD+.

Physical form

Lyophilized powder containing potassium phosphate buffer salt, EDTA, and DL-dithiothreitol

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Phytosterol ester processing in the small intestine; impact on cholesterol availability for absorption and chylomicron cholesterol incorporation in healthy humans
Amiot MJ, et al.
Journal of Lipid Research, jlr-M013730 (2011)
Pascal Barat et al.
Endocrinology, 148(10), 4836-4843 (2007-07-14)
Glucocorticoids are metabolized by 11beta-hydroxysteroid dehydrogenase 1 (11betaHSD1) and the A-ring reductases (5alpha- and 5beta-reductases). Dysregulation of these enzymes has been reported in liver and adipose tissue in obese humans and animals, potentially leading to altered intracellular glucocorticoid concentrations and
Bin Li et al.
Proteins, 71(2), 670-683 (2007-11-03)
Experimentally determined protein tertiary structures are rapidly accumulating in a database, partly due to the structural genomics projects. Included are proteins of unknown function, whose function has not been investigated by experiments and was not able to be predicted by
Chiaki Hidai et al.
Bioprocess and biosystems engineering, 32(5), 569-573 (2008-11-20)
Developing methods that result in targeting of therapeutic molecules in gene therapies to target tissues has importance, as targeting can increase efficacy and decrease off target-side-effects. Work from my laboratory previously showed that the extracellular matrix protein Del1 is organized
Expression and characterization of recombinant type 2 3alpha-hydroxysteroid dehydrogenase (HSD) from human prostate: demonstration of bifunctional 3alpha17beta-HSD activity and cellular distribution
Lin HK, et al.
Molecular Endocrinology, 11(13), 1971-1984 (1997)
View All Related Papers

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service