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TGP 55

95062833, mouse pancreas, Polymorph

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About This Item

UNSPSC Code:
41106514

product name

TGP 55, 95062833

biological source

mouse pancreas

growth mode

Adherent

karyotype

Not specified

morphology

Polymorph

products

Somatostatin and Insulin

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse pancreatic small cell anaplastic carcinoma

Cell Line Description

TGP 55 has been obtained from a small cell anaplastic carcinoma arising in the pancreas of a transgenic Tg(Ela-1SV40E)Bri 18 mouse these mice carry a pseudogene construct composed of a pancreatic-specific elastase-1 promoter linked to the coding region for SV40 T antigen and thereby represent an animal model for pancreatic carcinogenesis. TGP 55 cells are SV40T antigen positive and secrete somatostatin, it is one of a series of cell lines isolated from pancreatic tumours deposited at ECACC. Cells secrete somatostatin and insulin in culture. Adherent clumps of small cells.

Application

Study of exocrine pancreas carcinomas, hormone secretion studies.

Culture Medium

DMEM:Ham′s F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) (Heat Inactivated).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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Certificates of Analysis (COA)

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