A3401

Ala-Ala-Phe-7-amido-4-methylcoumarin

protease substrate

• Synonym(s): H-Ala-Ala-Phe-AMC
• Empirical Formula (Hill Notation): C₂₅H₂₈N₄O₅
• CAS Number: 62037-41-6
• Molecular Weight: 464.51
• MDL number: MFCD00070149
• UNSPSC Code: 12352204
• PubChem Substance ID: 24890766
• NACRES: NA.32

Properties

• Assay: ≥98% (TLC)
• form: powder
• solubility: ethanol: 20 mg/mL, clear, colorless to light yellow
• storage temp.: 2-8°C
• SMILES string: CC(N)C(=O)NC(C)C(=O)NC(Cc1ccccc1)C(=O)Nc2ccc3C(C)=CC(=O)Oc3c2
• InChI: 1S/C25H28N4O5/c1-14-11-22(30)34-21-13-18(9-10-19(14)21)28-25(33)20(12-17-7-5-4-6-8-17)29-24(32)16(3)27-23(31)15(2)26/h4-11,13,15-16,20H,12,26H2,1-3H3,(H,27,31)(H,28,33)(H,29,32)
• InChI key: FVRLYIFIDKXFHU-UHFFFAOYSA-N

Description

General description

Ala-Ala-Phe-7-amido-4-methylcoumarin is a fluorogenic substrate for the proteolytic activity. It is a positively charged substrate molecule.

Application

Ala-Ala-Phe-7-amido-4-methylcoumarin has been used:

• in the preparation of the reaction mixture for enzymatic assays
• to initiate the enzyme reaction in tripeptidyl peptidase-1 (TPP1) enzyme activity assay
• to initiate the assay reaction in lysosomal hydrolases activity assay

Substrates

Substrate for chymotrypsin and tripeptidyl peptidaseII.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves