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T3260

Sigma-Aldrich

Toluidine Blue O

Powder

Synonym(s):

Basic Blue 17, Blutene chloride, Methylene Blue T50 or T extra, Tolonium chloride

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About This Item

Empirical Formula (Hill Notation):
C15H16ClN3S
CAS Number:
Molecular Weight:
305.83
Colour Index Number:
52040
EC Number:
MDL number:
UNSPSC Code:
12171500
PubChem Substance ID:
NACRES:
NA.47

product name

Toluidine Blue O, Technical grade

grade

technical grade

Quality Level

form

powder

composition

Dye content, ~80%

solubility

H2O: 1 mg/mL

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

[Cl-].Cc1cc2N=C3C=CC(\C=C3Sc2cc1N)=[N+](\C)C

InChI

1S/C15H15N3S.ClH/c1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;/h4-8,16H,1-3H3;1H

InChI key

GEDVVYWLPUPJJZ-UHFFFAOYSA-N

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General description

Toluidine Blue O (TBO) is a thiazine dye of the quinone-imine family and is cationic in nature. It has been employed in polychromatic staining of paraffin embedded plant cell walls. It is a metachromatic dye suitable for a wide variety of histological staining procedures.
A metachromatic dye suitable for a wide variety of histological staining procedures.

Application

Toluidine Blue O has been used to stain a variety of specimens like mesenchymal cells, taste buds, osteoblasts, matrix, etc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Kyungho Yoon et al.
Ultrasound in medicine & biology, 45(9), 2391-2404 (2019-06-21)
Transcranial application of focused ultrasound (FUS) combined with vascular introduction of microbubble contrast agents (MBs) has emerged as a non-invasive technique that can temporarily create a localized opening in the blood-brain barrier (BBB). Under image-guidance, we administered FUS to sheep
Teaching Plant Anatomy Through Creative Laboratory Exercises null
An investigation of cellular dynamics during the development of intramembranous bones: the scleral ossicles
J. Jabalee
Journal of Anatomy (2013)
Polychromatic staining of plant cell walls by toluidine blue O
T.P.O'Brien
Protoplasma, 59(2) (1964)
The canine epiphyseal-derived mesenchymal stem cells are comparable to bone
marrow derived-mesenchymal stem cells
Ya-Pei CHANG
Surgery (2015)

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