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74303

Millipore

Legionella Agar Base

suitable for microbiology, NutriSelect® Plus

Synonym(s):

BCYE Agar without L-cysteine, Base

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About This Item

UNSPSC Code:
41171607
NACRES:
NA.85

sterility

non-sterile

Quality Level

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 13 g/L
charcoal activated, 2 g/L
yeast extract, 10 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

6.9±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
water monitoring

microbiology

suitability

nonselective for Legionella spp.

Application

Legionella Agar Base with the addition of supplement is used for the presumptive identification of Legionella species.

Preparation Note

Suspend 12.5 grams in 450 ml distilled water. Heat to boiling to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add contents of 1 vial of Legionella BCYE Supplement without L-Cysteine (Cat. No. 43753).

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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A W Pasculle et al.
The Journal of infectious diseases, 141(6), 727-732 (1980-06-01)
Pittsburgh pneumonia agent (PPA) was recently cultivated from infected egg yold on charcoal yeast extract agar. PPA has now been isolated both from infected egg yolk and human lung tissue on charcoal yeast extract agar and on a new medium
P H Edelstein
Journal of clinical microbiology, 14(3), 298-303 (1981-09-01)
Eighteen types of media, all of which used charcoal yeast extract medium as a base, were tested for their ability to support the growth of a stock strain of Legionella pneumophila. Fifteen of these contained antimicrobial agents. Five antibiotic-containing media
J C Feeley et al.
Journal of clinical microbiology, 10(4), 437-441 (1979-10-01)
Charcoal-yeast extract agar is a new bacteriological medium that supports excellent growth of the Legionella pneumophila. It results from modifications made in an existing L. pneumophila medium, F-G agar. Yeast extract, instead of an acid hydrolysate of casein, serves as
J C Feeley et al.
Journal of clinical microbiology, 8(3), 320-325 (1978-09-01)
Yolk sac suspensions infected with the Legionnaires disease bacterium (LDB) were plated onto 17 different bacteriological agar media. The LDB grew only on Mueller-Hinton agar supplemented with 1% Iso Vitale X and 1% hemoglobin (MH-IH). This medium was subsequently analyzed

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