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Sigma-Aldrich

Fiske-Subbarow reducer

Synonym(s):

Fiske-Subbarow reagent

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About This Item

MDL number:
UNSPSC Code:
12352302
NACRES:
NA.25

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General description

formulation of 1-amino-2-naphthol-4-sulfonic acid, sodium sulfite and bisulfite according to C.H. Fiske and Y. Subbarow, J.Biol.Chem. 66, 375 (1925)

Application

The Fiske-Subbarow assay is a colorimetric assay designed to measure levels of inorganic phosphate in biological samples. Fiske-Subbarow reducer reagent is used as the reducer component of the Fiske-Subbarow assay.

Pictograms

CorrosionExclamation mark

Signal Word

Danger

Hazard Statements

Hazard Classifications

Acute Tox. 4 Oral - Eye Dam. 1

Supplementary Hazards

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Eduardo H S Sousa et al.
Analytical biochemistry, 348(1), 57-63 (2005-11-18)
We describe the development of a rapid colorimetric assay for soluble guanylate cyclase (sGC) activity adapted for a 96-well microplate. The assay greatly decreases the analysis time and cost over traditional methodologies based on radio- and immunoassays and high-performance liquid
E Durward et al.
BioTechniques, 25(4), 608-612 (1998-10-30)
Methods for detecting and measuring the success of nucleic acid sequence amplifications can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method
A Kyaw et al.
Analytical biochemistry, 145(2), 230-234 (1985-03-01)
A new colorimetric procedure is described for inorganic phosphate determination using the color reaction between inorganic phosphate and acidified ammonium molybdate in the presence of Triton X-100. The method is simple and specific, and produces results comparable with those of
The colorimetric determination of phosphorus
Fiske, C. H., Subbarow, Y.
The Journal of Biological Chemistry, 66, 375-375 (1925)

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