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SCC065

Sigma-Aldrich

HL-1 Cardiac Muscle Cell Line

Mouse

Synonym(s):

HL-1 Cardiomyocyte Cell Line, HL-1 Atrial Muscle Cell Line

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About This Item

UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81

product name

HL-1 Cardiac Muscle Cell Line, HL-1 Cardiac Muscle Cell Line has been extensively characterized and is a valuable model system to address questions of cardiac biology at the cellular & molecular levels.

biological source

mouse

Quality Level

technique(s)

cell based assay: suitable
cell culture | mammalian: suitable

General description

HL-1 Cardiac Muscle Cell Line is an immortalized mouse cardiomyocyte cell line able to continuously divide and spontaneously contract while maintaining a differentiated cardiac phenotype. HL-1 can be serially passaged without losing its differentiated cardiac myocyte phenotype, including morphological, biochemical, and electrophysiological properties. HL-1 cells have been used to study normal cardiomyocyte function with regard to signaling, electrical, metabolic, and transcriptional regulation as well as to address pathological conditions such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis, and ischemia-reperfusion.

The HL-1 line was derived from AT-1 subcutaneous tumor excised from an adult female C57BL/6J mouse. The parental AT-1 line was originally derived from an atrial tumor growing in a transgenic mouse in which expression of the SV40 large T-antigen was targeted to atrial cardiomyocytes via the atrial natriuretic factor (ANF) promoter.

Cell Line Description

Cardiomyocyte Cells

Application

Research Category
Cardiac

Cardiovascular Disease

Toxicity
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

Quality

• Each vial contains ≥ 1X106 viable cells.
• Cells tested negative for infectious diseases by the Mouse Essential CLEAR panel PCR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.
• Functional Assay: Fluo-8 calcium dye fluorescent based assay for detecting changes in intracellular calcium

Storage and Stability

HL-1 Cardiac Muscle Cell Line should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges {HCompany} to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Jian Hou et al.
Brazilian journal of cardiovascular surgery, 34(6), 711-722 (2019-09-24)
To determine the role of the dishevelled binding antagonist of beta catenin 1 (DACT1) in the cytoskeletal arrangement of cardiomyocytes in atrial fibrillation (AF). The DACT1 expression and its associations with the degree of fibrosis and β-catenin in valvular disease
V Simonetti et al.
Evidence-based complementary and alternative medicine : eCAM, 2019, 2169103-2169103 (2019-12-13)
Skin reactions and cardiotoxicity are one of the most common side effects of doxorubicin in cancer patients. The main mechanisms based on the etiopathogenesis of these reactions are mediated by the overproduction of proinflammatory cytokines, metalloproteases, and the disruption of
Cora B Cox et al.
ImmunoHorizons, 6(6), 334-343 (2022-06-14)
Cardiac resident macrophages facilitate electrical conduction by interacting with cardiomyocytes via connexin-43 (Cx43) hemichannels. Cx43 is critical for impulse propagation and coordination between muscle contractions. Cardiomyocyte electrophysiology can be altered when coupled with noncardiomyocyte cell types such as M2c tissue-resident
Christine Quentin et al.
Molecules (Basel, Switzerland), 26(10) (2021-06-03)
Amlodipine, a unique long-lasting calcium channel antagonist and antihypertensive drug, has weak fluorescence in aqueous solutions. In the current paper, we show that direct visualization of amlodipine in live cells is possible due to the enhanced emission in cellular environment.
Yaxin Chen et al.
Journal of experimental & clinical cancer research : CR, 41(1), 1-1 (2022-01-05)
Immune checkpoint inhibitor-related cardiotoxicity is one of the most lethal adverse effects, and thus, the identification of underlying mechanisms for developing strategies to overcome it has clinical importance. This study aimed to investigate whether microbiota-host interactions contribute to PD-1/PD-L1 inhibitor-related

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