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MABT1365

Sigma-Aldrich

Anti-Tropomyosin-1 Antibody, clone 7F8B8

clone 7F8B8, from rat

Synonym(s):

Tropomyosin alpha-1 chain, Alpha-tropomyosin, Tropomyosin-1

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.43

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

7F8B8, monoclonal

species reactivity

human, mouse

packaging

antibody small pack of 25 μL

technique(s)

immunocytochemistry: suitable
western blot: suitable

isotype

IgG2aκ

NCBI accession no.

UniProt accession no.

target post-translational modification

unmodified

Gene Information

human ... TPM1(7168)

General description

Tropomyosin alpha-1 chain (UniProt: P09493; also known as Alpha-tropomyosin, Tropomyosin-1) is encoded by the TPM1 (also known as C15orf13, TMSA) gene (Gene ID: 7168) in human. Tropomyosin-1 is a cytoskeletal protein that associates with F-actin stress fibers. It binds to actin filaments in muscle and non-muscle cells and plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. It stabilizes actin filaments and regulates the interaction of actin filament with other actin-binding proteins. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Tropomyosin-1 is a homodimeric protein, but can form heterodimer of an alpha (TPM1, TPM3, or TPM4) and a beta (TPM2) chain. Mutations in TPM1 gene have been linked to cardiomyopathy characterized by ventricular hypertrophy and ventricular dilation and impaired systolic function that results in congestive heart failure and arrhythmia. Ten different isoforms of Tropomyosin-1 have been described that are produced by alternative splicing.

Specificity

Clone 7F8B8 detects human and murine Tropomyosin-1. It targets an epitope with in the N-terminal region.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptides corresponding to 24 amino acids from the N-terminal region of isoform 5 and 4 amino acids from the N-terminal region of isoform 2 of human tropomyosin-1.

Application

Anti-Tropomyosin-1, clone 7F8B8, Cat. No. MABT1365, is a rat monoclonal antibody that detects Tropomyosin-1 and has been tested for use in Immunocytochemistry and Western Blotting.
Immunocytochemistry Analysis: A representative lot detected Tropomyosin-1 in Immunocytochemistry applications (Brayford, S., et. al. (2016). Curr Biol. 26(10):1312-8).
Research Category
Cell Structure

Quality

Evaluated by Western Blotting in mouse embryonic fibroblast (MEF) lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Tropomyosin-1 in mouse embryonic fibroblast (MEF) lysate.

Target description

~30 kDa observed; 28.38 kDa for isoform 5. Uncharacterized bands may be observed in some lysate(s).

Physical form

Format: Purified
Protein G purified
Purified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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