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MAB1083

Sigma-Aldrich

Anti-GFP Antibody, clone 3F8.2

clone 3F8.2, from mouse

Synonym(s):

Green Fluorescent protein

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

3F8.2, monoclonal

species reactivity

vertebrates

technique(s)

western blot: suitable

isotype

IgG1κ

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

General description

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light.

Specificity

Reacts with most common vertebrate proteins containing the GFP tag.
The antibody reacts with the 28 kDa GFP protein.

Immunogen

Epitope: Full Length
Full length GFP protein.

Application

Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB.
Research Category
Secondary & Control Antibodies
Research Sub Category
Epitope Tags
Western Blot Analysis:
0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).

Quality

Routinely evaluated by Western Blot on GFP recombinant protein.

Target description

28 kDa

Physical form

Format: Purified
Protein G Purified
Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide.

Storage and Stability

Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
Western Blot: GFP recombinant protein

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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