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ABE1318

Sigma-Aldrich

Anti-SMARCD2 (BAF60B)

from rabbit, purified by affinity chromatography

Synonym(s):

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2, 60 kDa BRG-1/Brm-associated factor subunit B, BRG1-associated factor 60B, BAF60B

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

species reactivity (predicted by homology)

rat (based on 100% sequence homology), bovine (based on 100% sequence homology), mouse (based on 100% sequence homology)

technique(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

ambient

target post-translational modification

unmodified

Gene Information

human ... SMARCD2(6603)

General description

SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 (UniProt: Q92925; also known as 60 kDa BRG-1/Brm-associated factor subunit B, BRG1-associated factor 60B, BAF60B) is encoded by the SMARCD2 (also known as BAF60B, PRO2451) gene (Gene ID: 6603) in human. It is a member of the SWI/SNF family of proteins, whose members display helicase and ATPase activities and regulate transcription of certain genes by altering the chromatin structure around those genes. Three members of BAF60 family have been described and are designated as FAF60 A, B, and C. BAF60B is expressed in immune cells, muscle, and pancreas tissues. Two isoforms of BAF60B have been described that are produced by alternative splicing. The SWIB domain of BAF60B is localized in amino acids 307-382. BAF60B is reported to interact with multiple transcription factors and may bridge transcription factors with SWI/SNF complexes. At least 11 subunits of SWI/SNF complex have been discovered, including 2 enzymatic ATPase subunits (Brm and Brg1) and 10 Brg1/Brm associated factors (BAFs), which are named according to their molecular weights. BAF60B is shown to link chromatin opening to ATM activation by facilitating ATM recruitment to the open chromatin regions of a panel of hepatic gene loci. It is required for 3TF (transcription factors Foxa3, Hnf1 and Gata4) - induced activation of ATM and facilitates the recruitment of ATM to the SWI/ SNF complex. (Ref.: Ji, S., et al. (2017). Cell Res. 27(5); 642-656; Lores, P., et al. (2010). FEBS. J. 277(6); 1453-1464).

Specificity

This rabbit polyclonal antibody detects SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 (SMARCD2) in human cells. It targets an epitope within 13 amino acids from the N-terminal region.

Immunogen

Epitope: N-terminus
KLH-conjugated linear peptide corresponding to 13 amino acids from the N-terminal region of human SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 (BAF60B) protein.

Application

Anti-SMARCD2 (BAF60B), Cat. No. ABE1318, is a highly specific rabbit polyclonal antibody that targets SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily D member 2 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotti
Research Category
Epigenetics & Nuclear Function
Western Blotting Analysis: 0.5 µg/mL from a representative lot detected SMARCD2 (BAF60B) in 10 µg of HeLa cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected SMARCD2 (BAF60B) in HeLa, A431, HUVEC and NIH/3T3 cell lines.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected SMARCD2 (BAF60B) in human cerebral cortex, human testis, and human ovarian cancer tissue sections.

Quality

Evaluated by Western Blotting in Jurkat nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected SMARCD2 (BAF60B) in 10 µg of Jurkat cell nuclear extract.

Target description

~59 kDa observed; 58.92 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Physical form

Affinity Purified
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Certificates of Analysis (COA)

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