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Key Documents

AB1952

Sigma-Aldrich

Anti-Integrin beta1 Antibody, Cytosolic

serum, Chemicon®

Synonym(s):

CD29

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, rat, pig, human

manufacturer/tradename

Chemicon®

technique(s)

immunoprecipitation (IP): suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... ITGB1(3688)

General description

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Specificity

Reactive with human, mouse and rat integrin β-1, as confirmed by western blot. Not reactive with other integrins.

Immunogen

Epitope: C-terminus
Synthetic peptide corresponding to the c-terminal cytoplasmic domain of human β-1 integrin.

Application

Anti-Integrin beta1 Antibody, Cytosolic detects level of Integrin beta1 & has been published & validated for use in IP & WB.
Immunoprecipitation:
1 μL of antiserum per 25 μg total cell protein (in 40 μL). ) 0.5% triton X-100 lysis buffer with proteinase inhibitors is suggested.

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins

Quality

Western Blot Analysis:
1:500 to 1:1000 dilution of this antibody was used for detection of integrin beta1 in whole cell lysate under non-reducing. Size of beta 1 integrin under non-reduced conditions is approximately 110 kDa; often a 220 kDa dimer is observed as well; beta -1 under reduced conditions runs as a 130kDa band with higher molecular weight aggregates as well.

Target description

~130 kDa

Physical form

Rabbit polyclonal serum containing no preservatives
Unpurified

Storage and Stability

Stable for 1 year at -20ºC from date of receipt.

Analysis Note

Control
U251 cell line

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Rab11 and its effector Rab coupling protein contribute to the trafficking of beta 1 integrins during axon growth in adult dorsal root ganglion neurons and PC12 cells.
Eva, R; Dassie, E; Caswell, PT; Dick, G; ffrench-Constant, C; Norman, JC; Fawcett, JW
The Journal of Neuroscience null
Evgeny A Zemskov et al.
PloS one, 6(4), e19414-e19414 (2011-05-11)
Although endosomal compartments have been suggested to play a role in unconventional protein secretion, there is scarce experimental evidence for such involvement. Here we report that recycling endosomes are essential for externalization of cytoplasmic secretory protein tissue transglutaminase (tTG). The
Iwona Majkowska et al.
The Journal of biological chemistry, 292(16), 6633-6643 (2017-03-09)
Membrane-type 1 matrix metalloproteinase (MT1-MMP) is a membrane-bound MMP that is highly expressed in cells with invading capacity, including fibroblasts and invasive cancer cells. However, pathways of MT1-MMP up-regulation are not clearly understood. A potential physiological stimulus for MT1-MMP expression
Francesca Ricciardiello et al.
Cell death & disease, 9(3), 377-377 (2018-03-09)
Cancer aberrant N- and O-linked protein glycosylation, frequently resulting from an augmented flux through the Hexosamine Biosynthetic Pathway (HBP), play different roles in tumor progression. However, the low specificity and toxicity of the existing HBP inhibitors prevented their use for
Tomoyuki Okumura et al.
Oncogene, 22(26), 4017-4026 (2003-06-25)
We report here that human esophageal keratinocyte stem cells are characterized by the expression of the low-affinity neurotrophin receptor p75(NTR) and differentially expressed cell adhesion molecules, the beta1 and beta4 integrins. The candidate stem cells could be fractionated from keratinocytes

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