699500P

Kdo2-Lipid A (KLA)

Avanti Research^™ - A Croda Brand

• Synonym(s): KLA
• CAS Number: 1246298-62-3
• UNSPSC Code: 12352211
• NACRES: NA.25

Properties

• description: Di[3-deoxy-D-manno-octulosonyl]-lipid A (ammonium salt)
• Assay: >99% (HPLC)
• form: powder
• packaging: pkg of 1 × 1 mg (699500P-1mg)
• manufacturer/tradename: Avanti Research^™ - A Croda Brand
• application(s): lipidomics
• lipid type: bioactive lipids
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: O[C@H]([C@@H](CO[C@@H]([C@@H]1NC(C[C@H](OC(CCCCCCCCCCC)=O)CCCCCCCCCCC)=O)O[C@H](CO[C@]2(C([O-])=O)C[C@@H](O[C@@]3(C([O-])=O)O[C@H]([C@H](O)CO)[C@H](O)[C@H](O)C3)[C@@H](O)[C@@H]([C@H](O)CO)O2)[C@@H](OP([O-])(O)=O)[C@@H]1OC(C[C@H](OC(CCCCCCCCCCCCC)=O)CCCCCC

Description

General description

A new preparation of the saccharolipid glycan, Kdo2-Lipid A (KLA), is a nearly homogeneous Re lipopolysaccharide (LPS) substructure with endotoxin activity equal to that of native LPS. KLA is comparable to LPS and its activity is reduced by >103 in cells from TLR-4 deficient mice. The advantage of KLA over LPS is that it is a reproducible, defined natural product, and it can be detected by ESI/MS at the low concentrations used to stimulate animal cells. The purity of KLA should also facilitate the structural analysis of its complexes with signaling receptors, such as TLR-4/MD2. New research* shows 1μg KLA induces the same response as 10μg LPS.Perform experiments at half the cost of using LPS! 

Kdo2-Lipid A is a natural endotoxin and a combination of oligosaccharide and lipid.

Application

Kdo2-Lipid A (KLA) has been used to study its role in lipopolysaccharide induced prion protein conversion.

Kdo2-Lipid A (KLA) has been used:

• as a toll like receptor (TLR) ligand source in immortalized murine macrophage culture media
• in ²H-,¹³C-,¹⁵N-labeled OprHΔL1ΔL4 in 1,2-dihexanoyl-sn-glycero-3-phosphocholine (DHPC) micelles, to determine the specific residues of OprHΔL1ΔL4 interacting with lipopolysaccharides (LPS) source
• as a component of LPS stock to study its role in lipopolysaccharide induced prion protein conversion

Biochem/physiol Actions

Kdo2-Lipid A might be useful to stimulate inflammatory response.

Packaging

2 mL Amber Glass Crimp Cap Vial (699500P-1mg)

Other Notes

For R&D use only. Not for drug, household, or other uses.

Legal Information

Avanti Research is a trademark of Avanti Polar Lipids, LLC

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): No data available
• Flash Point(C): No data available