505013

Discovery^® RP-Amide C16 (5 µm) HPLC Columns

L × I.D. 15 cm × 4.6 mm, HPLC Column

• UNSPSC Code: 41115700
• eCl@ss: 32110501

Properties

• product name: Discovery^® RP-AmideC16 HPLC Column, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
• Agency: suitable for USP L60
• Quality Level: 100
• feature: endcapped
• manufacturer/tradename: Discovery^®
• extent of labeling: 11% Carbon loading
• parameter: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• technique(s): HPLC: suitable
• L × I.D.: 15 cm × 4.6 mm
• surface area: 200 m²/g
• surface coverage: 2.6 μmol/m²
• impurities: <10 ppm metals
• matrix: silica gel, high purity, spherical base material
• matrix active group: amide, alkyl phase
• particle size: 5 μm
• pore size: 180 Å
• operating pH range: 2-8
• application(s): food and beverages
• separation technique: reversed phase

Description

General description

Discovery^® RP-AmideC16 HPLC Column consists of a stationary phase made up of high-purity C₁₆-silica that includes a polar-embedded amide function attached to the silica surface with a propyl group.

Application

Discovery RP-AmideC16 HPLC Column may be used for the separation of:

• Impurities in drugs such as closantel, levamisole and azaconazole during their determination by high performance liquid chromatography-diode array detection (HPLC-DAD).
• Vitamin B₆ compounds in food samples during their determination using LC coupled with fluorescence detection (FLD).
• Vitamin C and other fat soluble vitamins in mature seeds of Lathyrus L. taxa during their identification using HPLC with ultraviolet (UV) detection.
• Zidovudine in combination with other pharmaceuticals in intravenous fluid mixtures during the determination using HPLC combined with photodiode array (PDA) detector.

Discovery^® RP-AmideC16 HPLC column may be used for the separation of:

• Phenolic compounds, purine alkaloids and theanine in Camellia species by high performance liquid chromatography (HPLC) equipped with photodiode array detector (PDA).
• Proprietary pharmaceutical compounds using HPLC combined with computer-assisted screening and Plackett−Burman experimental design.
• Hydrophilic bases by LC with principal component analysis (PCA).
• 2,4-Dinitrophenylhydrazone derivatives by HPLC equipped with ultraviolet (UV) spectrometric detection.

Features and Benefits

• Excellent retention and resolution of polar compounds
• Unique selectivity compared to C18
• Excellent peak shape and efficiency
• Less hydrophobic than C18 columns
• Compatible with 100% aqueous mobile phases

   

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Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable