SAB4200684

Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat

clone DECMA-1, purified from hybridoma cell culture

• Synonym(s): Arc-1, CD324, CDHE, E-cadherin (epithelial), ECAD, LCAM, UVO, cadherin 1, type 1
• UNSPSC Code: 12352209
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 200
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: DECMA-1, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~120 kDa
• species reactivity: dog, mouse
• concentration: ~1 mg/mL
• technique(s): immunoblotting: 0.5-1 μg/mL using whole extract of MDCK cells.; immunofluorescence: 5-10 μg/mL using MDCK cells; immunoprecipitation (IP): suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: mouse ... Cdh1(12550)

Description

General description

Monoclonal Anti-Uvomorulin/E-Cadherin (rat IgG1 isotype) is derived from the DECMA-1 hybridoma produced by the fusion of rat myeloma cells and splenocytes from an immunized Lou rat. Uvomorulin/E-Cadherin (Epithelial cadherin), also known as CDH1, CAM 120/80, L-CAM (Cell-Adhesion Molecule) or CD324, is a member of the cadherin transmembrane glycoproteins superfamily. It is characterized with a highly conserved cytoplasmic tail.

Immunogen

Mouse embryonal carcinoma cell line PCC4 Aza RI

Application

Monoclonal Anti-Uvomorulin/E-Cadherin antibody produced in rat has been used in various immunochemical techniques including immunoblotting, immunoprecipitation, immunohistochemical staining and immunofluorescent staining.

Biochem/physiol Actions

Cadherins contribute to epithelial cell behavior and tissue morphogenesis/remodeling by facilitating cell-cell adhesion. Monoclonal Anti-Uvomorulin/E-Cadherin can be used for studies of embryonal development, cell-cell interaction of cells grown in culture, and localization of E-Cadherin. In addition, it is commonly used as an E-cadherin inhibitor/blocking antibody in a study of embryonic stem cells (EMS).

E-cadherin is a calcium-dependent glycoprotein. It is responsible for the homogeneous attachment between cells. It suppresses malignant transformation of tumors. Additionally, it also regulates cellular invasion and metastasis.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable