S5394
Lipoprotein Deficient Serum from fetal calf
frozen liquid
Synonym(s):
Fetal Calf Serum, Lipoprotein Deficient Serum
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.61
Recommended Products
biological source
bovine fetus
Quality Level
form
frozen liquid
concentration
≥14 mg protein/mL Biuret
technique(s)
cell culture | mammalian: suitable
cell culture | stem cell: suitable
impurities
<1 ng/mg protein endotoxin
<5 % of normal lipoprotein
shipped in
dry ice
storage temp.
−20°C
General description
Studies show that the production of sterols is reduced in human fibroblasts cultured in the presence of lipoprotein deficient serum.
Application
Lipoprotein Deficient Serum from fetal calf has been used as a supplement in culture media to grow Chinese hamster ovary (CHO)-derived cells, thymic lobes and human peripheral blood mononuclear cells (PBMCs).
Preparation Note
Aseptically filtered, 0.2 μm
Dialyzed against Dulbecco′s phosphate buffered saline, pH 7.4-7.5
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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P M Giles et al.
Clinica chimica acta; international journal of clinical chemistry, 113(2), 183-191 (1981-06-18)
The biosynthesis and efflux of sterols from cells into the medium were investigated in skin fibroblasts from a control, a patient with obligate heterozygous familial hypercholesterolaemia and a patient with the homozygous condition. The behaviour of the cells was studied
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Lipid-mediated presentation of MHC class II molecules guides thymocytes to the CD4 lineage
Komaniwa S, et al.
European Journal of Immunology, 39(1), 96-112 (2009)
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Coenzyme Q (CoQ) is a lipid-like mobile electron transporter of the mitochondrial respiratory chain. Patients with partial loss-of-function mutations in the CoQ biosynthesis pathway suffer from partial primary CoQ deficiency (MIM 607426). This leads to mitochondrial dysfunction, which presents like
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