P9577
PCR 50 - 2,000 bp Marker
for electrophoresis of PCR fragments
Synonym(s):
50-2000 bp ladder, PCR ladder for agarose gel electrophoresis, PCR marker for agarose gel electrophoresis, RTU PCR DNA ladder, RTU PCR DNA marker, ready to use DNA ladder, ready to use DNA marker
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About This Item
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Quality Level
form
liquid
usage
sufficient for 50 uses
suitability
suitable for electrophoresis (DNA)
storage temp.
−20°C
General description
Sigma′s PCR 50 - 2,000 bp Marker contains 8 dsDNA fragments from 50 – 2,000 bases, evenly spaced. Five μl of the ladder can be loaded directly in a single lane on an agarose or polyacrylamide gel.
Application
PCR 50 - 2,000 bp Marker has been used as a marker for DNA gel electrophoresis.
Components
Sigma′s PCR 50 - 2,000 bp Marker is provided as two tubes:
- 1 vial (250 μl volume) PCR Marker
- 1 vial 6× loading buffer (250 μl volume) for diluting samples
Other Notes
Contains 8 bands, 50–2,000 bp in predictably spaced (ladder) double-stranded DNA fragments.
For optimal resolution, the recommended agarose gel concentration is 2.0%.
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Storage Class Code
10 - Combustible liquids
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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