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N6658

Sigma-Aldrich

Nutrient Mixture F-12 Ham

With ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):

Ham’s F-12

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About This Item

MDL number:
MFCD00217417
UNSPSC Code:
12352207
NACRES:
NA.75

product name

Nutrient Mixture F-12 Ham, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Quality Level

400

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

L-glutamine: yes
HEPES: no
phenol red: yes
sodium pyruvate: 0.11 g/L
NaHCO3: yes

shipped in

ambient

storage temp.

2-8°C

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General description

Ham′s Nutrient Mixtures were mainly created to support the clonal growth of numerous clones of Chinese hamster ovary (CHO) cells and clones of HeLa and mouse L-cells. It is also used to grow primary rat hepatocytes and rat prostate epithelial cells. Ham′s F-12 is used as the medium of choice for a clonal toxicity assay using CHO cells.

Application

Nutrient Mixture F-12 Ham has been used:
  • to culture Chinese hamster ovary cells overexpressing insulin receptor (CHO-IR)
  • to culture LAN-1 cells
  • to maintain SUM149, SUM159, SUM190, FC-IBC02, BCX010, and IBC3 cells
  • with Dulbecco’s modified Eagle’s medium (DMEM) media in a 1:1 ratio to culture oral cavity squamous cell carcinoma (OSCC) cell lines.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Heidi Olzscha et al.
Cell chemical biology, 24(1), 9-23 (2016-12-19)
Lysine acetylation is becoming increasingly recognized as a general biological principle in cellular homeostasis, and is subject to abnormal control in different human pathologies. Here, we describe a global effect on amyloid-like protein aggregation in human cells that results from
Ali I Mohammed et al.
Biomolecules, 13(8) (2023-08-26)
Reactive oxygen species (ROS) are highly reactive molecules generated in living organisms and an excessive production of ROS culminates in oxidative stress and cellular damage. Notably, oxidative stress plays a critical role in the pathogenesis of a number of oral
Chloe M Falvey et al.
Oncotarget, 8(14), 23479-23491 (2017-02-12)
Esophageal cancer remains a poor prognosis cancer due to advanced stage of presentation and drug resistant disease. To understand the molecular mechanisms influencing response to chemotherapy, we examined genes that are differentially expressed between drug sensitive, apoptosis competent esophageal cancer
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