N6288

Anti-Nucleolin−Atto 488 antibody produced in rabbit

1.5-3.0 mg/mL, affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-NCL, Protein C23
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: Atto 488 conjugate
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• species reactivity: human, mouse, rat
• storage condition: protect from light
• concentration: 1.5-3.0 mg/mL
• technique(s): direct immunofluorescence: 0.5-1.0 μg/mL using human HeLa, rat NRK and mouse 3T3 cells
• fluorescence: λ_ex 500 nm; λ_em 522 nm in PBS; λ_ex 500 nm; λ_em 522 nm
• UniProt accession no.: P19338
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... NCL(4691) ; mouse ... Ncl(17975) ; rat ... Ncl(25135)

Description

General description

Nucleolin (NCL) is an anti-apoptotic phosphoprotein. It is highly expressed in the nucleolus as well as other sub-cellular organelles.
Find more information here

Nucleolin is a multi-domain phosphoprotein mapped to human chromosome 2q37. It comprises three domains and a bipartite nuclear localization signal sequence. Nucleolin domains include the highly acidic N-terminal region, a RNA-binding central domain and a glycine, arginine and phenylalanine residues rich C-terminal region.

Specificity

Anti-Nucleolin-Atto 488 recognizes human, rat, and mouse nucleolin.

Immunogen

synthetic peptide corresponding to amino acid residues 2-17 of human nucleolin conjugated to KLH. The corresponding sequence differs by 4 amino acids in rat and mouse nucleolin, and by one amino acid in the rat homolog protein NRP (nucleolin related protein).

Application

Anti-Nucleolin−Atto 488 antibody produced in rabbit has been used in immunocytochemistry and immunofluorescence.

Biochem/physiol Actions

Nucleolin (NCL) interacts with proteins participating in microtubule nucleation. It takes part in the transcription of polymerase I, formation of ribosomes and duplication of centrosomes. NCL is also a transcription regulator. Studies have shown that it is highly expressed in many tumors.

Nucleolin N- and C-terminal domains are involved in protein-protein interaction, whereas the central domain is involved in specific interactions with nucleic acids. Nucleolin exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin′s multiple activities may be regulated by proteolysis and methylation. It also interacts with telomerase andthis interaction is critical for the nucleolar localization of telomerase. Nucleolin might function as a cell surface receptor for lipoproteins, viruses, extracellular matrix, growth factors and other molecules.

Physical form

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves