MSP13
Membrane Scaffold Protein 1D1 BTN
recombinant, expressed in E. coli, Biotin labeled
Synonym(s):
1D1 BTN Protein, Membrane Protein Scaffold, Scaffold Protein 1D1 BTN
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About This Item
UNSPSC Code:
12352200
NACRES:
NA.26
Recommended Products
recombinant
expressed in E. coli
Assay
≥90% (SDS-GE)
form
buffered aqueous solution
mol wt
26,900.1 Da by calculation (without biotin)
solubility
water: soluble
shipped in
ambient
storage temp.
−20°C
General description
Research area: Cell Struc
The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence. The MSP1D1 BTN variant of MSP1D1 features an enzymatically biotinylated additional 20-amino acid sequence at the C-terminus of MSP1D1.
Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1)
The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The Membrane Scaffold Protein 1D1 (MSP1D1) variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the accompanying figure) of the original MSP1 sequence. The MSP1D1 BTN variant of MSP1D1 features an enzymatically biotinylated additional 20-amino acid sequence at the C-terminus of MSP1D1.
Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1)
Application
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.
Biochem/physiol Actions
Scaffold proteins are vital for imparting specificity to the mitogen-activated protein kinase (MAPK) pathway, which is necessary for normal cellular functions and development. They assist in regulating the localization of MAPK components, enabling precise modulation of cellular responses while preserving overall MAPK activity.
Legal Information
Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
- 7,691,414 Membrane scaffold proteins
- 7,662,410 Membrane scaffold proteins and embedded membrane proteins
- 7,622,437 Tissue factor compositions and methods
- 7,592,008 Membrane scaffold proteins
- 7,575,763 Membrane scaffold proteins and tethered membrane proteins
- 7,083,958 Membrane scaffold proteins
- 7,048,949 Membrane scaffold proteins
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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