MITOISO1
Mitochondria Isolation Kit
sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues
Synonym(s):
Mitochondria Purification Kit
Sign Into View Organizational & Contract Pricing
All Photos(2)
About This Item
Recommended Products
Quality Level
usage
sufficient for 10-20 g (animal tissue)
sufficient for 50 assays (2 mL)
shelf life
2 yr
technique(s)
fractionation: suitable
shipped in
dry ice
storage temp.
−20°C
Looking for similar products? Visit Product Comparison Guide
Application
Mitochondria Isolation Kit has been used:
- for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
- for the isolation of mitochondria from glomeruli and heart
- for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential
The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.
Features and Benefits
- Specially formulated extraction reagents & proven procedure suitable for small scale isolation - Isolate an enriched, intact mitochodrial fraction in a microfuge tube.
- Produces functionally active, intact mitochondria - Resulting mitochondria are suitable for in vitro assays for apoptosis, oxidative stress or other studies.
- Includes stain & protocol for determining inner membrane integrity - Determine the integrity of the inner mitochondrial membrane without the need to purchase other reagents.
- Compatible with the Cytochrome c Oxidase Assay Kit - Allows easy determination of the integrity of the outer mitochondrial membrane
Kit Components Also Available Separately
Product No.
Description
SDS
- T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solidSDS
related product
Product No.
Description
Pricing
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Customers Also Viewed
Young-Kyoung Shin et al.
Cancer research, 65(8), 3162-3170 (2005-04-19)
5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
Mitochondrial dysfunction mediates aldosterone-induced podocyte damage: a therapeutic target of PPARgamma
Zhu C, et al.
The American Journal of Pathology, 178(5), 2020-2031 (2011)
Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties
Palzur E, et al.
Brain Research, 1221, 126-133 (2008)
Takehiko Dohi et al.
The Journal of clinical investigation, 114(8), 1117-1127 (2004-10-19)
Evasion of apoptosis is a hallmark of cancer, but the molecular circuitries of this process are not understood. Here we show that survivin, a member of the inhibitor of apoptosis gene family that is overexpressed in cancer, exists in a
Jacinta Serpa et al.
The Journal of biological chemistry, 285(50), 39211-39223 (2010-10-12)
The short chain fatty acid (SCFA) butyrate is a product of colonic fermentation of dietary fibers. It is the main source of energy for normal colonocytes, but cannot be metabolized by most tumor cells. Butyrate also functions as a histone
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service