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MAK040

Sigma-Aldrich

Adipogenesis Kit

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Triglyceride Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.25

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric
fluorometric

relevant disease(s)

endocrinological disorders, diabetes; cardiovascular diseases; cancer

storage temp.

−20°C

General description

Adipogenesis is the differentiation of pre-adipocytes derived from pluripotent mesenchymal cells into mature adipocytes. Adipocytes, which are capable of storing large quantities of lipids as triglycerides, are the main cells for lipid storage in animals. Adipose tissue plays a critical role in the regulation of whole-body energy homeostasis and adipocyte dysfunction has been implicated in pathological states such as cancer, type II diabetes, cardiovascular disease, and atherosclerosis.

Omental adipocytes are known to promote the invasion and progression of cancer cells especially, ovarian cancer. Close proximity of tumor cells and adipocytes, causes a phenotypic change resulting in the formation of adipocyte-derived fibroblasts, which directly promotes tumor progression. Elevated level of adiponectin produced by adipocytes is observed in heart failure patients, cardiovascular disease, and atherosclerosis. Excess cytokines such as TNFα (tumor necrosis factor -α) in adipocytes results in chronic inflammatory state and inhibits the process of adipogenesis. Adipocytes are known to maintain glucose homeostasis. Adipocytes regulates the entry of fatty acids into circulation and skeletal muscle. This action of adipocytes is associated with the development of type 2 diabetes, which indicates insulin resistance as a result of high levels of fatty acids in the blood.

Application

Adipogenesis Kit has been used to study the intramyocellular triglyceride accumulation in myotubes. It has been used to study the effect of NS5ABP37 on hepatocellular carcinoma by determining the total intracellular triglyceride.

Suitability

Suitable for the quantification of triglyceride accumulation in cells and tissue

Principle

In this kit, total cellular and tissue concentrations of triglycerides are determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the triglycerides present. This kit is able to detect triglycerides in as few as 1 × 104 differentiated 3T3 L1 cells.

Pictograms

Health hazardCorrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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A new role for the natriuretic peptides: metabolic regulators of the adipocyte.
Costello-Boerrigter L C and Burnett J C
Journal of the American College of Cardiology (2009)
Adipocytes promote ovarian cancer metastasis and provide energy for rapid tumor growth.
Nieman K M, et al.
Nature Medicine, 17(11), 1498-1498 (2011)
Adipocyte dysfunctions linking obesity to insulin resistance and type 2 diabetes.
Guilherme A, et al.
Nature Reviews in Molecular and Cell Biology, 9(5), 367-367 (2008)
Adipocyte-Derived Fibroblasts promote tumor progression and contribute to desmoplastic reaction in breast cancer.
Bochet L, et al.
Cancer Research, 530-530 (2013)
Chien-Liang Fang et al.
Frontiers in pharmacology, 9, 155-155 (2018-03-22)
The dermis of human skin contains large numbers of fibroblasts that are responsible for the production of the extracellular matrix (ECM) that supporting skin integrity, elasticity and wound healing. Previously, an in vivo study demonstrated that dermal fibroblasts siting in

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