M3148

2-Mercaptoethanol

for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

• Synonym(s): β-Mercaptoethanol, 2-Hydroxyethylmercaptan, BME, Thioethylene glycol
• Linear Formula: HSCH₂CH₂OH
• CAS Number: 60-24-2
• Molecular Weight: 78.13
• Beilstein: 773648
• EC Number: 200-464-6
• MDL number: MFCD00004890
• UNSPSC Code: 12161504
• PubChem Substance ID: 24896800
• NACRES: NA.31

Properties

• grade: for molecular biology
• Quality Level: 200
• vapor density: 2.69 (vs air)
• vapor pressure: 1 mmHg ( 20 °C)
• product line: BioReagent
• Assay: 99% (GC/titration)
• form: liquid
• expl. lim.: 18 %
• reaction suitability: reagent type: reductant
• concentration: 14.3 M (pure liquid)
• technique(s): cell culture | mammalian: suitable; electrophoresis: suitable
• refractive index: n20/D 1.500 (lit.)
• pH: 4.5-6 (20 °C, 500 g/L)
• bp: 157 °C (lit.)
• solubility: H₂O: soluble 1 mL/mL
• density: 1.114 g/mL at 25 °C (lit.)
• foreign activity: DNase, RNase, protease, none detected
• storage temp.: 2-8°C
• SMILES string: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

Description

Application

Reducing agent
β-mercaptoethanol or 2-mercaptoethanol has been used:

• as a supplement in Roswell Park Memorial Institute (RPMI)-1640 medium to culture DT40 cells (chicken B cell line)
• to lyse NuLi-1 (Normal Lung, University of Iowa-1) cells
• to trypsinize mouse embryonic stem cells

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

Safety Information

• Pictograms: GHS06,GHS08,GHS05,GHS09
• Signal Word: Danger
• Hazard Statements: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• Precautionary Statements: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• Target Organs: Liver,Heart
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point(F): 154.4 °F - closed cup
• Flash Point(C): 68 °C - closed cup
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves