G2751

Glutamic-Oxalacetic Transaminase from porcine heart

Type I, ammonium sulfate suspension, 200-500 units/mg protein

• Synonym(s): L-Aspartate:2-oxoglutarate aminotransferase, Aspartate Aminotransferase, GOT
• CAS Number: 9000-97-9
• Enzyme Commission number: 2.6.1.1 (BRENDA, IUBMB)
• EC Number: 232-571-9
• MDL number: MFCD00131191
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• type: Type I
• Quality Level: 200
• form: ammonium sulfate suspension
• specific activity: 200-500 units/mg protein
• foreign activity: glutamic-pyruvic transaminase ≤0.03%; lactic dehydrogenase ≤0.01%; malic dehydrogenase ≤0.01%
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Research area: Cellsignaling. Glutamic-Oxalacetic Transaminase is found in all tissues and is predominant in the liver and skeletal muscle. It is a prototype of fold-type I pyridoxal 5′-phosphate (PLP)-enzymes. Glutamic-oxalacetic transaminase is a homodimer containing large and small domains in each subunit.

Application

Glutamic-OxalaceticTransaminase from porcine heart is suitable for the preparation ofphenylalanine amino acid. The immobilized form of this enzyme maybe used incomparison studies between soluble and immobilized forms in terms of reactionefficiency and stability.

Glutamic-Oxalacetic Transaminase from porcine heart has been used:

• in an enzyme-coupled assay for evaluating L-asparaginase enzyme activity
• in microtiter plate format enzyme-based assay to estimate malic acid content in berry samples
• as a supplement in homogenization buffers for the isolation of heart mitochondrial membranes in the presence of oxaloacetate (OAA)-depleting system

Biochem/physiol Actions

Glutamic-oxalacetic transaminase or aspartate aminotransferase (AAT) catalyzes the interconversion of L-aspartate and α-ketoglutarate with oxalacetate and L-glutamate. This reversible transaminase reaction is dependent on pyridoxal 5′-phosphate (PLP). Glutamic-oxalacetic transaminase maintains the nitrogen currency for metabolism by generating L-glutamate. Elevated serum levels of glutamic-oxalacetic transaminase are indicated in myocardial infarction, liver diseases, and some renal diseases.

Unit Definition

One unit will convert 1.0 μmole of α-ketoglutarate to L-glutamate per min at pH 7.5 at 37 °C, in the presence of L-aspartic acid. One unit is equivalent to ~2,000O.D. (Karmen) units at 25 °C.

Physical form

Suspension in 3.0 M (NH₄)₂SO₄ containing 0.05 M maleate and 2.5 mM α-ketoglutarate, pH 6.0

Analysis Note

Protein determined by biuret.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves