D8187
JumpStart™ REDTaq® DNA Polymerase
Hot-start Taq enzyme with inert dye, 10X buffer included
Synonym(s):
Hot start DNA polymerase, Hot start Taq
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About This Item
Recommended Products
form
liquid
usage
sufficient for 250 reactions
sufficient for 2500 reactions
sufficient for 50 reactions
feature
dNTPs included: no
hotstart
concentration
1 unit/μL
technique(s)
PCR: suitable
color
red
input
purified DNA
suitability
suitable for PCR
shipped in
wet ice
storage temp.
−20°C
General description
JumpStart™ REDTaq® DNA Polymerase is Sigma′s high performance Taq DNA Polymerase blended with JumpStart Taq antibody and an inert red dye tracer. Extensive testing with a variety of primers and templates indicates that the performance of JumpStart REDTaq DNA Polymerase is equivalent to, or better than, that of standard Taq polymerase.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
Since the red tracer has no effect on the amplification process, a sample can be easily re-amplified such as in “nested PCR”. The presence of the dye also has no effect on automated DNA sequencing; ligase mediated ligations, exonucleolytic PCR product digestion, and transformation. Though exceptions may exist, the dye is generally inert in restriction enzyme digestions. If necessary, the dye can be removed from the amplicon by routine purification methodologies.
Application
JumpStart™ REDTaq® DNA Polymerase has been used in polymerase chain reaction (PCR) for the amplification of:
- insulin-enterotoxin ricin fusion gene (INS-RTB)
- endothelial cells DNA derived from reverse transcribed RNA
- leg genomic DNA from cricket flies
- mitochondrial gene by conventional PCR
Features and Benefits
- Reduces non-specific amplification
- Increased target yield and specificity
- Higher the amplification irrespective of the target concentration
- Reduce set-up time and eliminate concerns associated with manual or wax hot start methods
- Visual confirmation that the enzyme has been added and that proper component mixing of the reaction has occurred
- Samples can be loaded directly onto an agarose gel for electrophoresis without loading buffers or tracking dyes
- Assembled PCR reactions can be placed at room temperature for up to 2 hours
Packaging
The enzyme is provided with an optimized 10× reaction buffer.
Unit Definition
One unit incorporates 10 nmol of total dNTPs into acid-precipitable DNA in 30 min. at 74 °C.
Other Notes
View more detailed information on JumpStart REDTaq enzymes at www.sigma-aldrich.com/hotstart.
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: US 8,404,464 and US 7,972,828. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims.
JumpStart is a trademark of Sigma-Aldrich Co. LLC
REDTaq is a registered trademark of Merck KGaA, Darmstadt, Germany
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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