C3138
Cathepsin D from bovine spleen
lyophilized powder, ≥2.0 units/mg protein
Synonym(s):
CTSD
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.32
Recommended Products
biological source
bovine spleen
Quality Level
Assay
10—70% protein (biuret)
form
lyophilized powder
specific activity
≥2.0 units/mg protein
mol wt
~45 kDa
manufacturer/tradename
Sigma-Aldrich
technique(s)
activity assay: suitable
color
dark brown
suitability
suitable for molecular biology
UniProt accession no.
application(s)
life science and biopharma
storage temp.
−20°C
Gene Information
cow ... CTSD(282883)
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General description
Research area: Cell Signaling
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Cathepsin D is a soluble endosomal-lysosomal aspartic protease and is synthesized in the rough endoplasmic reticulum as preprocathepsin D. It is encoded by the CTSD gene located in the 11p15.5 region.
Application
Cathepsin D from bovine spleen has been used:
- in in vitro dose-dependent fluorometric activity assays.
- in in vitro myelin oligodendrocyte glycoprotein (MOG) digestion to study the uptake of malondialdehyde (MDA)-modified MOG and its implications in central nervous system autoimmunity.
- for enzymatic digestion of the proteoglycan moiety of the articular cartilage in order to determine its dynamic elastic modulus at two different levels of tissue organization.
- in cathepsin D activity assay.
Biochem/physiol Actions
Cathepsin D is an endosomal-lysosomal aspartic protease implicated in breast cancer metastasis and Alzheimer′s disease. Lysosomal release of cathepsin D has been found to precede cytochrome c release and loss of membrane potential in apoptotic human foreskin fibroblasts. Cathepsin D levels in PC12 cells increase 12 to 24 hours after apoptosis is induced.
Unit Definition
One unit will produce an increase in A280 of 1.0 per min per mL at pH 3.0 at 37 °C measured as TCA-soluble products using hemoglobin as substrate (1 cm light path).
Physical form
Lyophilized powder containing citrate buffer salts
inhibitor
related product
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Martin Stolz et al.
Biophysical journal, 86(5), 3269-3283 (2004-04-28)
Cartilage stiffness was measured ex vivo at the micrometer and nanometer scales to explore structure-mechanical property relationships at smaller scales than has been done previously. A method was developed to measure the dynamic elastic modulus, |E(*)|, in compression by indentation-type
Olja Mijanovic et al.
Pharmaceutics, 13(6) (2021-07-03)
Lysosomal proteases play a crucial role in maintaining cell homeostasis. Human cathepsin D manages protein turnover degrading misfolded and aggregated proteins and favors apoptosis in the case of proteostasis disruption. However, when cathepsin D regulation is affected, it can contribute
Cathepsin D--many functions of one aspartic protease
Benes P, et al.
Critical Reviews in Oncology/Hematology, 68(1), 12-28 (2008)
Marie Maynadier et al.
Journal of controlled release : official journal of the Controlled Release Society, 171(2), 251-257 (2013-08-01)
Implication of the intracellular proteolytic activity of Cathepsin D (CathD), a lysosomal aspartyl-protease overexpressed in numerous solid tumors, has been evidenced on tumor growth. Its intracellular inhibition by potent inhibitors such as pepstatin constitutes a relevant but challenging molecular target.
Yoko Shiba et al.
Current biology : CB, 23(19), 1945-1951 (2013-10-01)
ArfGAPs are known to be involved in cargo sorting in COPI transport. However, the role of ArfGAPs in post-Golgi membrane traffic has not been defined. To determine the function of ArfGAPs in post-Golgi traffic, we used small interfering RNA to
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