B4875

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride

trypsin substrate, chromogenic, elastase substrate, ≥98%, powder

• Synonym(s): N_α-Benzoyl-DL-arginine p-nitroanilide hydrochloride, BANI, BAPNA
• Empirical Formula (Hill Notation): C₁₉H₂₂N₆O₄ · HCl
• CAS Number: 911-77-3
• Molecular Weight: 434.88
• Beilstein: 4081878
• EC Number: 213-011-2
• MDL number: MFCD00012846
• UNSPSC Code: 12352204
• PubChem Substance ID: 24891801
• NACRES: NA.32

Properties

• product name: Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride, ≥98%
• Assay: ≥98%
• form: powder
• color: , white to light yellow to light brown
• solubility: DMSO: 50 mg/mL (colorless to dark green-yellow to dark brown-yellow)
• storage temp.: −20°C
• SMILES string: Cl[H].NC(=N)NCCCC(NC(=O)c1ccccc1)C(=O)Nc2ccc(cc2)[N+]([O-])=O
• InChI: 1S/C19H22N6O4.ClH/c20-19(21)22-12-4-7-16(24-17(26)13-5-2-1-3-6-13)18(27)23-14-8-10-15(11-9-14)25(28)29;/h1-3,5-6,8-11,16H,4,7,12H2,(H,23,27)(H,24,26)(H4,20,21,22);1H
• InChI key: DEOKFPFLXFNAON-UHFFFAOYSA-N

Description

General description

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride (DL-BAPNA) is a chromogenic substrate for proteolytic enzymes such as trypsin, amidase, and balterobin. Hydrolysis of DL-BAPNA at the bond between the arginine and the p-nitroaniline moieties releases the chromophore p-nitroaniline, which can be detected by colorimetric analysis.

Application

Nα-Benzoyl-DL-arginine 4-nitroanilide hydrochloride has been used as a substrate for trypsin and elastase in enzyme analysis.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrates

A chromogenic trypsin substrate.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves