62302

Lipase from Candida rugosa

lyophilized, powder (fine), 15-25 U/mg

• Synonym(s): CCL
• CAS Number: 9001-62-1
• Enzyme Commission number: 3.1.1.3 (BRENDA, IUBMB)
• EC Number: 232-619-9
• MDL number: MFCD00131509
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: fungus (Candida rugosa)
• Quality Level: 100
• form: lyophilized solid; powder (fine)
• quality: lyophilized
• specific activity: 15-25 U/mg
• mol wt: M_r ~67000
• storage temp.: 2-8°C
• InChI: 1S/C11H9N3O2.Na/c15-8-4-5-9(10(16)7-8)13-14-11-3-1-2-6-12-11;/h1-7,16H,(H,12,14);/q;+1/b13-9-;
• InChI key: QWZUIMCIEOCSJF-CHHCPSLASA-N

Description

General description

Research area: Cell signaling

Lipase is a part of the alpha/beta-hydrolase fold superfamily of enzymes and is expressed in multiple tissues, including the liver, adipocytes, small intestine, and endothelial surface.

Application

Lipase from Candida rugosa has been used:

• for the resolution of chiral compounds and the transesterification production of biodiesel.
• for studying the immobilization of different lipase enzymes on graphene oxide support for high-efficiency biocatalysis.

Biochem/physiol Actions

Candida rugosa lipase is known to catalyze hydrolysis reactions, especially the production of ricinoleic acid.

Lipases catalyze the hydrolysis of triacylglycerols into glycerol and free fatty acids.

Unit Definition

1 U corresponds to the amount of enzyme which liberates 1 μmol oleic acid per minute at pH 8.0 and 40 °C (triolein, Cat. No. 62314 as substrate)

Other Notes

A highly stereospecific catalyst suitable for preparative resolution of racemic acids and alcohols; Stereoselective ester synthesis; Benzyl-alkyl transesterification under mild neutral conditions; Enantioselectivity of some lipases: Review

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves