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SE100

Sigma-Aldrich

Silver Enhancer Kit

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About This Item

UNSPSC Code:
12171500
NACRES:
NA.47

form

liquid

Quality Level

usage

sufficient for ≥100 slides staining and fixing

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

Application

Silver Enhancer Kit has been used to determine the presence of gold nanorods in various tissues and to prepare silver enhanced gold colloids for electron microscopy.

Features and Benefits

Colloidal gold labels are normally visible only at the electron microscope level. Silver Enhancer enlarges the gold colloid label by precipitation of metallic silver to give a high contrast signal visible under a light microscope. A darkroom is not necessary for this procedure, as the silver enhancer working solution is light-stable for 20-30 minutes under ordinary laboratory lighting.

Packaging

The Silver Enhancer kit consists of Solution A (silver salt), Solution B (initiator), Sodium Thiosulfate (fixer), and a Technical Bulletin describing their use.

Principle

Solution A and Solution B are mixed 1:1 immediately before use and applied to the slide holding the gold-labeled section. After 5-10 minutes, the slide is rinsed with distilled water, fixed for 2-3 minutes in a sodium thiosulfate solution, and rinsed again. The section is now ready for counterstaining and mounting as required.

Kit Components Only

Product No.
Description

  • Sodium thiosulfate pentahydrate, reagent grade, ≥99.5% 500 g

Kit Components Also Available Separately

Product No.
Description
SDS

  • S5020Silver enhancer solution A 100 mLSDS

  • S5145Silver enhancer solution B 100 mLSDS

Pictograms

Health hazardEnvironment

Signal Word

Danger

Hazard Statements

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Repr. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Yuan Chen et al.
Oncotarget, 9(41), 26556-26571 (2018-06-15)
Tumor hypoxia is a well-recognized driver of resistance to traditional cancer therapies such as chemotherapy and radiation therapy. We describe development of a new nanoconstruct composed of gold nanorods (GNRs) conjugated to carbonic anhydrase IX (CAIX) antibody that specifically binds
Joanna Seiffert et al.
Respiratory research, 17(1), 85-85 (2016-07-21)
The increasing use of silver nanoparticles (AgNPs) in consumer products is concerning. We examined the potential toxic effects when inhaled in Brown-Norway (BN) rats with a pre-inflammatory state compared to Sprague-Dawley (SD) rats. We determined the effect of AgNPs generated
Catarina Coutinho et al.
Analytical and bioanalytical chemistry, 411(9), 1807-1824 (2018-11-06)
MicroRNAs (miRNAs) are small regulatory RNAs, the dysregulation of which has been associated with the progression of several human diseases, including cancer. Interestingly, these molecules can be used as biomarkers for early disease diagnosis and can be found in a
F Liu et al.
The Plant cell, 13(5), 1063-1078 (2001-05-08)
Some plant cytoplasms express novel mitochondrial genes that cause male sterility. Nuclear genes that disrupt the accumulation of the corresponding mitochondrial gene products can restore fertility to such plants. The Texas (T) cytoplasm mitochondrial genome of maize expresses a novel
Using Immuno-Scanning Electron Microscopy
for the Observation of Focal Adhesion-substratum
interactions at the Nano- and Microscale in S-Phase Cells
Manus J.P. Biggs
Cell Culture Labfax (2011)

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