11333062910
Roche
Anti-Digoxigenin
from mouse IgG1κ (clone 1.71.256)
Synonym(s):
anti-digoxigenin, digoxigenin
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
clone 1.71.256, monoclonal
form
lyophilized
packaging
pkg of 100 μg
manufacturer/tradename
Roche
technique(s)
ELISA: 2-4 μg/mL
immunocytochemistry: 0.5-2 μg/mL
immunohistochemistry: 0.5-2 μg/mL
western blot: 0.5-2 μg/mL
isotype
IgG1κ
storage temp.
2-8°C
General description
Contents: Lyophilizate
Digoxigenin is a hapten, useful in labeling nucleic acids and in detection systems. Probes labeled with digoxigenin has greater sensitivity equivalent to that of radioactive probes, allows faster detection. It is less hazardous and has increased shelf life.
Specificity
The monoclonal antibody reacts with free and bound digoxigenin.
Application
Use Anti-Digoxigenin antibody for the detection of digoxigenin-labeled componds using:
- ELISA
- Immunohistocytochemistry
- In situ hybridization
- Western blot
- Immunofluorescence staining
- FISH (fluorescent in situ hybridization)
Biochem/physiol Actions
In the presence of Na+, Mg2+ and adenosine triphosphate (ATP), digoxigenin inhibits sodium pumps.
Features and Benefits
- Specific to free and bound digoxigenin
- The antibody is not stabilized with protein and therefore suitable as coating antibody in Sandwich-ELISAs and for labeling procedures. For example it can be conjugated with enzymes or fluorescence dyes for direct detection of digoxigenin-labeled samples.
Preparation Note
Working concentration: Working concentration of antibody depends on application and substrate. The following concentrations should be taken as a guideline:
Working solution: For coating applications
phosphate buffered saline, pH 7.4
- ELISA: for coating: 2 to 4 μg/ml
- Immunohistocytochemistry: 0.5 to 2 μg/ml
- In situ hybridization: 0.2 to 0.4 μg/ml
- Western blot: 0.5 to 2 μg/ml
Working solution: For coating applications
phosphate buffered saline, pH 7.4
Reconstitution
Add 1 ml double-distilled water to a final concentration of 100 μg/ml.
Storage and Stability
The lyophilized antibody is stable at +2 to +8°C. The reconstituted antibody solution is stable up to 6 months at +2 to +8°C. The solution can be stored in aliquots at -15 to -25°C. Avoid repeated freezing and thawing.
Analysis Note
There are no cross reactivities known.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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