If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/449/386/product-dating-information-mk.pdf
753610
Gold nanoparticles
20 nm diameter, OD 1, stabilized suspension in 0.1 mM PBS, reactant free
Synonym(s):
Au NP, Gold colloid
About This Item
Recommended Products
form
nanoparticles
suspension
concentration
~6.54E+11 particles/mL
OD
1
diameter
20 nm
λmax
518-522 nm
PDI
<0.2
storage temp.
2-8°C
SMILES string
[Au]
InChI
1S/Au
InChI key
PCHJSUWPFVWCPO-UHFFFAOYSA-N
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General description
Application
Legal Information
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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Customers Also Viewed
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How can I determine the shelf life / expiration / retest date of this product?
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Helpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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If i want to concentrate or isolate gold mannan particles and remove any unlabeled mannan what filtration or centrifugation conditions should be used.
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This product is comprised of stabilized gold nanoparticles in 0.1 mM PBS. There is no mannan present. The material may be concentrated by centrifugation. Note that aggregation may become an issue if the particles are removed from the stabilizing solution.
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Why does my gold nanoparticle solution turn violet when I add salt containing buffer?
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Due to repulsive forces arising from the surface charge of gold nanoparticles, an energy barrier must be overcome for individual particles to interact. When no (or small) amounts of electrolytes such as NaCl is present, this energy barrier is too strong for interaction to occur between particles. However, upon addition of NaCl this energy barrier is reduced allowing the gold nanoparticles to interact and aggregate. This aggregation causes a phenomenon called surface-plasmon coupling which changes the adsorption maximum of light to a higher wavelength resulting in a change in color of the solution.
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How do you attach gold nanoparticles to glass?
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To view the procedure on how to attach gold nanoparticles to glass please view information in the reference - Journal of Atomic, Molecular, and Optical PhysicsVolume 2012 (2012). Article ID 683830, 6 pagesdoi:10.1155/2012/683830
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Which gold nanoparticle size should I choose for my application?
1 answer-
The size of gold nanoparticle to use is very depandent upon the intended application. Generally, smaller particles offer better sensitivity in applications such as immunogold labeling due to less steric hindrance and the ability to bind more gold nanoparticles to the desired target. Small gold nanoparticles are less visible than larger particles, however, which must also be taken into account.As an application example, particles with a size between 30-50nm are particularly useful for the development of rapid tests such as lateral flow assays.
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My gold nanoparticles settle out of solution upon storage. Is this normal?
1 answer-
Settling of gold nanoparticles on the bottom of the storage flask is completely normal and is especially common for larger sized particles, which settles at greater speed. Settling does not affect the performance of the particles. Prior to use, simply swirl the solution to properly disperse your gold nanoparticles and obtain a homogenous solution.
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How does the storage temperature relate to shipping conditions?
1 answer-
The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.
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How do you attach gold nanoparticles to oligos?
1 answer-
To attach gold nanoparticles to oligos please view the information in the attached reference - Bioconjug Chem. 2011 Apr 20;22(4):794-807. Epub 2011 Mar 28.
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How do you attach gold nanoparticles to peptides and peptide conjugates?
1 answer-
To view information on how to attach gold nanoparticles to peptides and peptide conjugates, please view the information in the reference - Rosenthal, S. J. and Wright, D. W. (Eds.). (2005), p. 91-92. NanoBiotechnology Protocols. New Jersey. Humana Press.
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