11024AST
Astec® CHIROBIOTIC® V Chiral (5 μm) HPLC Columns
L × I.D. 25 cm × 4.6 mm, HPLC Column
Sinónimos:
V Chiral Stationary Phase Column
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About This Item
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
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product name
Astec® Chirobiotic® V Chiral HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
material
stainless steel column
agency
suitable for USP L88
description
HPLC Column
product line
Astec®
packaging
pkg of 1 ea
manufacturer/tradename
Astec®
parameter
0-45 °C temperature
241 bar pressure (3500 psi)
technique(s)
HPLC: suitable
LC/MS: suitable
L × I.D.
25 cm × 4.6 mm
matrix
High-purity silica gel particle platform
fully porous particle
matrix active group
vancomycin phase
particle size
5 μm
pore size
100 Å
operating pH range
3.5-7.0
separation technique
chiral
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General description
Neutral molecules, amides, acids, esters and amines show considerable enantioselectivity on these vancomycin-based CSPs. A wide variety of secondary and tertiary amines have been separated on the CHIROBIOTIC® V in the polar ionic mode. The CHIROBIOTIC® V has demonstrated many of the separation characteristics of protein-based stationary phases, but with exceptional stability and much higher sample capacity. Some chiral analytes have been resolved that have not been reported separated on any other chiral stationary phase. CHIROBIOTIC® V and V2 differ in their bonding chemistry the pore size of the support particle, giving them different selectivity and preparative capacity.
- Bonded phase: Vancomycin
- Operating pH range: 3.5 - 7.0
- Particle diameter: 5, 10 or 16 μm
- Pore size: 100 Å (CHIROBIOTIC® V) or 200 Å (CHIROBIOTIC® V2)
Application
Astec® CHIROBIOTIC® V Chiral HPLC column may be used for the separation of different types of racemic compounds (ß-blockers, ß-agonists, benzodiazipines, non-steroidal anti-inflammatory drugs, barbiturates, free and derivatized amino acids in packed column subcritical fluid chromatography (pSFC). It may also be used for the separation of citalopram enantiomers and its two N-demethylated metabolites and of alprenolol that is the internal standard, in human plasma using chiral liquid chromatography. It may also be used in the chiral liquid chromatography analysis of ketoprofen and flurbiprofen.
Legal Information
Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC
Application
Referencia del producto
Descripción
Precios
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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F Péhourcq et al.
Biomedical chromatography : BMC, 15(3), 217-222 (2001-06-08)
Vancomycin is an amphoteric, glycopeptide, macrocyclic antibiotic. When attached to 5 microspherical silica gel, vancomycin proved to be an effective chromatographic chiral stationary phase that could be used in the reversed-phase mode. In this study, a bonded vancomycin chiral stationary
Chiral packed column subcritical fluid chromatography on polysaccharide and macrocyclic antibiotic chiral stationary phases.
Andrei M
Journal of Chromatography A, 785 (1), 159-171 (1997)
M Kosel et al.
Journal of chromatography. B, Biomedical sciences and applications, 719(1-2), 234-238 (1998-12-30)
A procedure using a chirobiotic V column is presented which allows separation of the enantiomers of citalopram and its two N-demethylated metabolites, and of the internal standard, alprenolol, in human plasma. Citalopram, demethylcitalopram and didemethylcitalopram, as well as the internal
Ana R Ribeiro et al.
Chemosphere, 95, 589-596 (2013-11-05)
Microbial degradation is the most important process to remove organic pollutants in Waste Water Treatment Plants. Regarding chiral compounds this process is normally enantioselective and needs the suitable analytical methodology to follow the removal of both enantiomers in an accurate
Enantiomeric separation of mirtazapine and its metabolite in rat plasma by reverse polar ionic liquid chromatography using fluorescence and polarimetric detectors connected in series
Rao, R. Nageswara, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 879 (21), 1911-1916 (2011)
Chromatograms
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