Saltar al contenido
Merck
Todas las fotos(10)

Documentos

ZRB1427

Sigma-Aldrich

Anti-Macrosialin/CD68 Antibody, clone 2L16 ZooMAb® Rabbit Monoclonal

enhanced validation
greener alternative

recombinant, expressed in HEK 293 cells

Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización


About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

rabbit

Quality Level

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

2L16, recombinant monoclonal

description

recombinant, expressed in HEK 293 cells

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 37.41 kDa
observed mol wt ~33 kDa

purified by

using Protein A

species reactivity

human

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

sustainability

Greener Alternative Product

technique(s)

ELISA: suitable
affinity binding assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

isotype

IgG

epitope sequence

N-terminal

Protein ID accession no.

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 2L16 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects human Macrosialin/CD68. It targets an epitope within 16 amino acids from the N-terminal, extracellular domain.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the N-terminal region of human Macrosialin/CD68.

Application

Quality Control Testing

Evaluated by Western Blotting in U937 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Macrosialin/CD68 in U937 cell lysate.

Tested applications

Affinity Binding Assay: A representative lot of this antibody bound CD68 with a KD of 2.6 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis): A 1:1,000 dilution from a representative lot detected Macrosialin/CD68 in human tonsil tissue sections.

ELISA Analysis: A representative lot detected Macrosialin/CD68 peptide.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Anti-Macrosialin/CD68, clone 2L16 ZooMAb®, Cat. No. ZRB1427, is a recombinant Rabbit monoclonal antibody that specifically targets of Macrosialin/CD68 and is used in Affinity Binding Assay, ELISA, Immunohistochemistry (Paraffin), and Western Blotting.

Target description

Macrosialin (UniProt: P34810; also known as Gp110, CD68) is encoded by the CD68 gene (Gene ID: 968) in human. CD68 is a single-pass type I membrane glycoprotein that is synthesized with a signal peptide (aa 1-21), which is subsequently cleaved off to generate the mature form that contains an extracellular domain (aa 22-319), a transmembrane domain (aa 320-345), and a short cytoplasmic tail (aa 345-354). It also contains a mucin-like domain (aa 23-140) and has two 30 amino acid long repeats (aa 70-99 and 100-129), proline-rich hinge (aa 140-152), and a LAMP-like domain. CD68 is highly expressed in blood monocytes and tissue macrophages. Its expression is also observed in lymphocytes, fibroblasts, and endothelial cells. It is also found in many tumor cells and allows them to attach to selectin on vascular endothelium, facilitating their dissemination to secondary sites. CD68 is mainly located in the endosomal/lysosomal compartment but can rapidly shuttle to the cell surface. In macrophages, it plays a role in their phagocytic activities, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. It binds to tissue- and organ-specific lectins or selectins, which allows for homing of macrophages to particular sites. It has a good predictive value as a prognostic marker of survival in cancer subjects. CD68 expression is shown to be upregulated in macrophages in response to inflammatory stimuli, such as exposure to oxLDL, lipopolysaccharide, or interferon-g. Hence, it can serve as a good histochemical/cytochemical marker of inflammation associated with the involvement of monocytes/macrophages. Its expression is not observed in dendritic cells; hence it can be used to immunohistochemically distinguish macrophages from dendritic cells. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Chistiakoc, DA., et al. (2017). Lab. Invest. 97(1); 4-13).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb® formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Not finding the right product?  

Try our Herramienta de selección de productos.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

¿Ya tiene este producto?

Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.

Visite la Librería de documentos

Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.

Póngase en contacto con el Servicio técnico