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Y2146

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without histidine, leucine, and tryptophan

Sinónimos:

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements, Yeast media, amino acid dropout supplements

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About This Item

Número MDL:
MFCD01095679
Código UNSPSC:
41106212
NACRES:
NA.85

Nivel de calidad

200

Formulario

powder

técnicas

transformation: suitable

aplicaciones

microbiology

temp. de almacenamiento

room temp

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Categorías relacionadas

Aplicación

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.
Yeast Synthetic Drop-out Medium Supplements are suitable for screening yeast transformants.

Otras notas

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Nota de preparación

Dissolve 1.46 g of supplement per liter of medium.

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Referencia del producto
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Y2001

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan and uracil

Y1376

Yeast Synthetic Drop-out Medium Supplements, without leucine

Y0626

Base de nitrógeno de levadura sin aminoácidos, Yeast classification medium used for selecting yeasts based on amino acid and carbohydrate requirements

Y1771

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan

Y1896

Yeast Synthetic Drop-out Medium Supplements, without lysine

Y2021

Yeast Synthetic Drop-out Medium Supplements, without histidine, leucine, tryptophan, and adenine

Pictogramas

Corrosion
GHS05

Palabra de señalización

Danger

Frases de peligro

H318

Consejos de prudencia

P280 - P305 + P351 + P338

Clasificaciones de peligro

Eye Dam. 1

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

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Certificados de análisis (COA)

Lot/Batch Number
0000342960
0000166949
0000206149
079M4161V

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Sudeshna Nag et al.
Journal of cell science, 131(18) (2018-08-30)
Sorting endosomes (SEs) are the regulatory hubs for sorting cargo to multiple organelles, including lysosome-related organelles, such as melanosomes in melanocytes. In parallel, melanosome biogenesis is initiated from SEs with the processing and sequential transport of melanocyte-specific proteins toward maturing
Riddhi Atul Jani et al.
Journal of cell science, 128(17), 3263-3276 (2015-07-26)
Melanosomes are a class of lysosome-related organelles produced by melanocytes. Biogenesis of melanosomes requires the transport of melanin-synthesizing enzymes from tubular recycling endosomes to maturing melanosomes. The SNARE proteins involved in these transport or fusion steps have been poorly studied.

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