U7883

Monoclonal Anti-USP25 antibody produced in mouse

~1.0 mg/mL, clone U25.7, purified immunoglobulin, buffered aqueous solution

• Sinónimos: Anti-USP21, Anti-Ubiquitin specific peptidase 25
• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• conjugado: unconjugated
• forma del anticuerpo: purified immunoglobulin
• tipo de anticuerpo: primary antibodies
• clon: U25.7, monoclonal
• Formulario: buffered aqueous solution
• mol peso: antigen ~126 kDa
• reactividad de especies: rat, mouse, human
• concentración: ~1.0 mg/mL
• técnicas: immunocytochemistry: suitable; indirect ELISA: suitable; western blot: 1-2 μg/mL using total extract of HEK-293T cells expressing USP25.
• isotipo: IgG2a
• Nº de acceso UniProt: Q9UHP3
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... USP25(29761) ; mouse ... Usp25(30940) ; rat ... Usp25(304150)

Descripción

Descripción general

Monoclonal Anti-USP25 (mouse IgG2a isotype) is derived from the hybridoma U25.7 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human USP25. USP25 (ubiquitin-specific protease 25) is a member of the ubiquitin processing proteases (UBP) family. It is located on the gene-poor region of 21q11.2 human chromosome.

Aplicación

Monoclonal Anti-USP25 antibody has been used in:

• enzyme linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry

Acciones bioquímicas o fisiológicas

USP25 (ubiquitin-specific protease 25) is a target for sumoylation. It takes place within the two ubiquitin interaction motifs (UIM) of USP25 that are required for efficient hydrolysis of Ub (ubiquitin), and thus impairs USP25 activity as a result of reduced affinity to Ub chains. Overexpression of USP25 has been observed in Down syndrome fetal brain.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)