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TDF100A

Kit de análisis de la fibra alimentaria total

sufficient for ~100 assays

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NACRES:
NA.84
UNSPSC Code:
12164500
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usage

sufficient for ~100 assays

Quality Segment

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

storage temp.

2-8°C

Application

Este ensayo permite determinar el contenido de fibra alimentaria total de los alimentos utilizando una combinación de métodos enzimáticos y gravimétricos. Las muestras de alimentos secos y sin grasa se gelatinizan con α-amilasa termoestable y luego se someten a digestión enzimática con proteasas y amiloglucosidasas para eliminar las proteínas y el almidón presentes en la muestra. Se añade etanol para precipitar la fibra alimentaria soluble. A continuación se filtra el residuo y se lava con etanol y acetona. Después de secar, se pesa el residuo. La mitad de las muestras se utilizan para determinar las proteínas y el resto se convierten en ceniza. La fibra alimentaria total es el peso del residuo menos el peso de las proteínas y la ceniza.

Biochem/physiol Actions

Utiliza una combinación de métodos enzimáticos y gravimétricos. Este procedimiento se basa en el método publicado en los Métodos oficiales de análisis (Official Methods of Analysis), 15 edición, AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990).

Other Notes

Reactivos para la determinación de fibra alimentaria total.

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Este artículo
TDFC10FA20GAHK20
description

sufficient for ~100 assays

description

sufficient for ≥10 assays

description

sufficient for 20 assays

description

sufficient for 20 assays

technique(s)

photometry: suitable

technique(s)

photometry: suitable

technique(s)

photometry: suitable

technique(s)

photometry: suitable

application(s)

food and beverages
general analytical

application(s)

food and beverages
general analytical

application(s)

food and beverages
general analytical

application(s)

food and beverages
general analytical

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

usage

sufficient for ~100 assays

usage

sufficient for ≥10 assays

usage

sufficient for 20 assays

usage

sufficient for 20 assays

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

microbialanalysis
microbialanalysis

microbialanalysis
microbialanalysis

microbialanalysis
microbialanalysis

microbialanalysis
microbialanalysis


Solo componentes del kit

Referencia del producto
Descripción

  • α-Amylase, Heat-stable 10 mL

  • Amyloglucosidase 10 mL

  • Celite 50 g

  • Protease 500 mg

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT RE 1 Inhalation - STOT SE 3

target_organs

Lungs, Respiratory system

Clase de almacenamiento

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

1–3 of 3 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. This Kit quantify solubles and insulubles fibers ?

    1 answer
    1. This kit is used only to quantify soluble dietary fibers.

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

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